Cisbio, Codolet, France
The proper optimization of assay conditions is essential to ensure you obtain the best reagent use and performance. In the case of Cell Signaling, the most suitable assay conditions are dependent on the signaling pathway being investigated as well on the pharmacological characteristics of the compound being studied (activator or inhibitor). The optimal assay conditions will also hinge on the particular cellular model used for the assay (adherent or suspension cells, immortalized cell line or primary cells, tumor, tissue…). For each setup, a number of optimization steps can be implemented that will ensure the best, most accurate results are obtained. Drawing on hundreds of data sets and cases established by Cisbio’s scientists over the years, our manual provides the scientific community with the most up to date guidelines for the optimization of Cell Signaling assays.