Viral double-stranded RNA detection Kit

It is proven that the generation of double-stranded RNA (dsRNA) during genome replication is a feature common to many viruses. Traditional methods for detecting virus infection have relied on titering virus particles (plaque assay). These methods have proven lengthy, often requiring several days. Cisbio's dsRNA assay detects the presence of dsRNA in cell lysates, a byproduct of viral replication activity. Using this method, results can be obtained the same day.

Assay principle

HTRF® - the homogeneous cell-based sandwich immunoassay

dsRNA is detected in a sandwich assay format using 2 antibodies, one labeled with Europium Cryptate (donor) and the second with d2 (acceptor). The detection principle is based on HTRF® technology. When the dyes are in close proximity, the excitation of the donor with a light source (laser or flash lamp) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor,  which in turn fluoresces at a specific wavelength (665 nm). The two antibodies bind to the dsRNA present in the sample, thus generating FRET. Signal intensity is proportional to the dsRNA concentration.

Viral double stranded rna detection kit assay principle

A simpler, more flexible assay protocol - adapted to your applications

The assay protocol, using a 384-well small volume white plate or a Cisbio low volume 96 well plate (20 µL final), is described on the right. 10µl of cell lysates or control are dispensed directly into the plate for detection by HTRF® reagents. The antibodies labeled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Viral double stranded rna detection kit assay protocol

Assay validation

1.Detection of synthetic dsRNA

Signal obtained for a range of concentrations of a synthetic dsRNA template diluted in the kit's lysis buffer. The synthetic dsRNA is used to mimic the dsRNA produced upon viral infection and subsequent genome replication.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Viral double-stranded RNA detection kit - 500 tests64RNAPEG
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Viral double-stranded RNA detection kit - 10,000 tests64RNAPEH
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Companion products

DescriptionCat. noProduct insertMSDS
Lysis buffer 4 - 16 ml64LB4FDD
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Lysis buffer 4 - 130 ml64LB4FDF
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Viral double-stranded RNA detection kit standard64RNATDA
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