It is proven that the generation of double-stranded RNA (dsRNA) during genome replication is a feature common to many viruses. Traditional methods for detecting virus infection have relied on titering virus particles (plaque assay). These methods have proven lengthy, often requiring several days. Cisbio's dsRNA assay detects the presence of dsRNA in cell lysates, a byproduct of viral replication activity. Using this method, results can be obtained the same day.
Our Technology HTRF
HTRF® technology principle:
Truly homogeneous functional assays
FRET (Fluorescence Resonance Energy Transfer) is based on the transfer of energy between two fluorophores – a donor and an acceptor – when in close proximity. Molecular interactions between biomolecules can be assessed by coupling each partner with a fluorescent label and by detecting the level of energy transfer.