Free and bound Insulin immunoradiometric assay kit RIA
The BI-INS-US kit is an immunoradiometric assay for a sensitive and specific measure of human insulin.
For Research Use Only, not for use in diagnostic procedures.
- HIGH SENSITIVITY
- NO CROSS-REACTION WITH PROINSULIN
Two anti-insulin monoclonal antibodies are used to form a sandwich. The first monoclonal antibody is adsorbed onto the tube walls, while the second is radiolabelled with iodine-125.
Two alternative assay procedures:
1- measurement of immunoreactive insulin (free insulin + insulin bound to anti-insulin antibodies): 2h at RT / shaking
2- measurement of free insulin: 15 min centrifugation + 2h at RT / shaking
Working range: 0 – 500 µIU/mL or 0 – 20 ng/mL (1st IRP 66/304) Sample: serum or plasma – 50 µL
Analytical sensitivity: 0.2 µIU/mL (8 pg/mL)