PAb Anti-phospho MEK1/2-Eu cryptate
Anti;phospho MEK1/2;Eu cryptateto detect phospho;MEK1/2;Ser 217/221.
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HIF-1a is one of the 2 subunits of the transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 acts as a master regulator of cellular and systemic response to hypoxia by activating the translation of genes involved in energy metabolism, angiogenesis, and apoptosis, among others. High levels of HIF-1 have been associated with aggressive tumor progression. HIF-1a is also strongly linked to diabetes through its involvement in the glucose uptake of target cells. It has also been implicated in diabetes complications, such as diabetic nephropathy or diabetic wound repair.
Human MCF-7 epidermoid carcinoma cells were plated at 50,000 cells/well in a 96 well plate. After a preincubation with increasing concentrations of 1mM DFO for 30 minutes to prevent the degradation of HIF-1α , the cells were treated for 6 h at 37°C with increasing concentrations of the PI3K inhibitor LY294002 for 30 minutes (in triplicate) to inhibit the PI3K/AKT pathway. The cells were then activated with 10 nM IGF-1 and 1mM DFO. Next, the medium was removed and the cells were lysed with 50 µL of supplemented lysis buffer#4 for 30 min at RT under gentle shaking. 16 µL of lysate were then transferred into 2 different wells of a 384-sv plate for measurement in parallel.
HIF1 consists of a constitutively expressed HIF-1ß subunit (87 kDa) and an O2-regulated HIF-1a subunit (120 kDa) which contains an O2-dependent degradation domain (ODD).
Under normoxia, HIF-1a is hydroxylated on its ODD domain by O2-activated prolyl hydroxylases (PHDs), leading to its rapid ubiquitination and subsequent degradation by the proteasome.
Under hypoxia, PHD activity is inhibited and HIF-1a accumulates in the cytosol, translocates to the nucleus, heterodimerizes with HIF-1ß, and is activated. The complex activates the transcription of more than 100 target genes that facilitate responses to the hypoxic environment by encoding proteins involved in angiogenesis, erythropoiesis, cell proliferation/survival, and inflammation.
Product Insert HIF-1 a Kit / 64HIFPEG-64HIFPEH
64HIFPEG-64HIFPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (ELL) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (ITA) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (SPA) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) HIF-1 a Kit / 64HIFPEG
64HIFPEG - Safety Data Sheet
Safety Data Sheet (DEU) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (ELL) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (ITA) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (SPA) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) HIF-1 a Kit / 64HIFPEH
64HIFPEH - Safety Data Sheet
Safety Data Sheet (DEU) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
Safety Data Sheet (ELL) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
Safety Data Sheet (ITA) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
Safety Data Sheet (SPA) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) HIF-1 a Kit / 64HIFPEJ
64HIFPEJ - Safety Data Sheet
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