Histamine H1R expressing cells labeled with Terbium

Tag-lite cells transiently expressing the Histamine H1 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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  • Fully functional cell line
  • Transient expression
  • Ready for binding
Tag-lite cells transiently expressing the Histamine H1 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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Overview

The histamine H1 receptors are distributed in several tissues, including the brain and the smooth muscles from airways. Stimulation of histamine H1 leads to the synthesis and release of several neurochemicals and neuromodulators.

Cells expressing the H1 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Assay principle

Running your H1 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using Histamine H1 labeled cells and their matching fluorescent ligand (L0015GRE). Doxepin was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

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Tag-lite binding assays

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Kinetics of the ß2 Adrenergic receptor binding and signaling

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Product Insert H1-R LabCell / C1TT1H1

C1TT1H1 Lot 09 - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

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Safety Data Sheet H1-R LabCell / C1TT1H1

C1TT1H1 - Safety Data Sheet

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