HIV p24 Detection Kit HTRF®
The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.
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No-wash
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Ease-of-use
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Enables fast assay qualification
Overview
Immunodeficiency Virus (HIV) p24 is the 231 amino acid phosphorylated protein of the capsid forming the conical core of the virus, which encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the p55 Gag polyprotein by viral proteases. HIV p24 and its 55 kDa precursor play crucial roles in the assembly, maturation, and disassembly of HIV. In gene therapy, HIV-derived lentiviral vectors are useful and commonly implemented to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.
Key features
16 µL of recombinant protein in diluent #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti HIVp24 detection reagents. The HTRF signal was recorded after 2h incubation at RT.
Sample size | 16 µL |
Final assay volume | 20 µL |
Time to results | 2h at RT |
Detection limit | 12 pg/mL |
Dynamic range | 33 to 10,000 pg/mL |
Sample compatibility | Cell supernatant |
How HTRF compares to Western Blot and ELISA
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Immunoassays RIA and ELISA - Catalog
See how researchers boost their gene therapy studies with PerkinElmer technologies - Application Notes
Product Insert HIV p24 Kit / 64P24PEG-64P24PEH
64P24PEG-64P24PEH - Product Insert
Safety Data Sheet (DEU) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (ELL) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (ITA) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (SPA) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) HIV p24 Kit / 64P24PEG
64P24PEG - Safety Data Sheet
Safety Data Sheet (DEU) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Safety Data Sheet (ELL) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Safety Data Sheet (ITA) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Safety Data Sheet (SPA) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) HIV p24 Kit / 64P24PEH
64P24PEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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