HTRF 384-well low volume white plate

These 384w low volume plates can be used with all HTRF assays and read on all HTRF certified readers

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These 384w low volume plates can be used with all HTRF assays and read on all HTRF certified readers

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In stock

Overview

These plates have been specifically designed by Cisbio. Cisbio’s 384-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy.

Plate characteristics

Format 384 well
Color White
Bottom Opaque
Maximum volume / well 32 µL
Recommended working volume / well 20-26 µL
Culture NOT culture treated
Lid Compatible
Sealer Compatible (and recommended)
Reader settings 384 half-area
Validation Inter-batch &intra-batch

HTRF assays in HTRF 384-well plate and in Greiner 384-well plate

Assay reagents were dispensed side-by-side in an HTRF 384-well low volume plate and in a Greiner 384-well low volume plate. As shown below, similar results were obtained, whether the assay was performed in Greiner plates or in HTRF plates.

1. cAMP Gs dymanic kit

A standard curve was prepared and dispensed in the two different plates: 10 µL of each standard, 5 µL of cAMP-d2 and 5 µL of anti cAMP- cryptate were added into the wells of each plate. HTRF signal was recorded after a 1 hour incubation at RT.

2. Mouse CCL2 kit

A standard curve was prepared and dispensed in the two different plates: 16 µL of each standard and 4 µL of pre-mixed Anti-CCL2 antibodies were added into the wells of each plate. HTRF signal was recorded after an overnight incubation at RT.

3. Total SMAD3 kit

16 µL of various amount of untreated NIH3T3 cells lysed with the supplemented lysis buffer #4, were dispensed into the wells of the two different plates, 4 µL of of pre-mixed Anti-SMAD3 antibodies were added into the wells of each plate. HTRF signal was recorded after an overnight incubation at RT.
Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

HTRF 96- and 384- well low volume plates

Specifically designed by Cisbio to be used with all HTRF assays - Flyers

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