Home
Life sciences
Phosphorylation from A to Z
Phosphorylation assays
HTRF Human and Mouse Phospho-HDAC4 Ser246 Detection Kit

HTRF Human and Mouse Phospho-HDAC4 Ser246 Detection Kit HTRF®

Associated products

This HTRF kit enables the cell-based quantitative detection of phosphorylated HDAC4 at Ser246.

All inclusive kit
Low sample consumption
No-wash
High sensitivity

Download the protocols

This HTRF kit enables the cell-based quantitative detection of phosphorylated HDAC4 at Ser246.

Size

Cat. number selected :

Quantity

Overview

Histone deacetylases (HDACs) regulate chromatin remodeling and subsequent gene transcription by controlling the status of histone acetylation. Histone deacetylation induces a condensed chromatin conformation, contributing to the repression of gene transcription, which is involved in diverse physiological processes.

Importantly, HDACs are dysregulated in a number of brain disorders. This is implicated in the pathogenesis of these diseases, autism, Alzheimer’s disease, and depressive disorders. It suggests that HDACs might be potential targets for the treatment of brain disorders.

Among class II HDACs , HDAC4 is a specific target for the treatment of ischemic stroke. It plays a key role in the pathogenesis of ischemic stroke, and also in post-stroke recovery by affecting neuronal death, angiogenesis, and neurogenesis.

Epigenetic pathways define biologically relevant subsets of human cancers. EZH2 activation and HDAC4 activation correlate with growth factor signaling and inflammation, respectively, and represent two distinct states for cancer cells. This understanding may enable us to identify targetable drivers in breast cancer and mesenchymal glioblastoma.

Benefits

SPECIFICITY
PRECISION
COMPOUND PROFILING

Phospho-HDAC4 (Ser246) assay principle

The Phospho-HDAC4 (Ser246) assay measures HDAC4 when phosphorylated at Ser246. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Phospho-HDAC4 (Ser246) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. The first antibody was selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. Protein phosphorylation enables an immune-complex formation involving both labeled antibodies that brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample, and provides a means of assessing the protein’s phosphorylation state under a no-wash assay format.

Principle of the HTRF Phospho HDAC4 assay

Phospho-HDAC4 (Ser246) 2-plate assay protocol

The 2 plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before adding Phospho-HDAC4 (Ser246) HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.

Two-plate protocol of the HTRF Phospho HDAC4 assay

Phospho-HDAC4 (Ser246) 1-plate assay protocol

Detection of Phosphorylated HDAC4(Ser246) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.

One-plate protocol of the HTRF Phospho HDAC4 assay

Key guidelines to successful cell signaling experiments

Mastering the art of cell signaling assays optimization - Guides

HTRF cellular phospho-protein assays

Physiologically relevant results fo fast flowing research - Flyers

Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences

Insider Tips for successful sample treatment - Technical Notes

Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Technical Notes

Best practices for analyzing tumor xenografts with HTRF phospho assays

Protocol for tumor xenograft analysis with HTRF - Technical Notes

HTRF® phospho-protein platform facilitates the dissection of signaling pathways involved in insulin resistance and metabolic disorders

Multi-tissue cellular modeling and anlysis of insulin signaling - Posters

HTRF® cell signaling platform combined with iCell® Hepatocytes

A solution for phospho-protein analysis in metabolic disorders - Posters

HTRF phospho-assays reveal subtle drug-induced effects

Detailed protocol and direct comparison with WB - Posters

Implement HTRF phospho-protein assays at every step of the drug discovery process, from in vitro to in vivo models

A single technology for 2D cells, 3D cells, and xenograft models - Posters

Simplify complex pathway dissection by combining the power of HTRF® cellular phospho-assays and the flexibility of the CyBi®-FeliX liquid handling system

PI3K/AKT/mTor translational control pathway - Posters

Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery

One technology across all samples - Application Notes

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

Tumor xenograft analysis: HTRF versus Western blot - Application Notes

HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Application Notes

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Application Notes

HTRF Alpha-tubulin Housekeeping kit

Properly interpret your compound effect - Application Notes

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Application Notes

How to run a cell based phospho HTRF assay

What to expect at the bench - Videos

Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening

In collaboration with Bayer - Scientific Presentations

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Videos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Videos

Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection

Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes

Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)

Learn how to reduce time and sample consumption - Application Notes

Assessment of drug efficacy and toxicity by combining innovative technologies

Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes

Product Insert HDAC4 p-S246 Kit / 64HDAC4S6PEG-64HDAC4S6PEH

64HDAC4S6PEG-64HDAC4S6PEH - Product Insert

Safety Data Sheet (DEU) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (ELL) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (ITA) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (SPA) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (ENG-US) HDAC4 p-S246 Kit / 64HDAC4S6PEG

64HDAC4S6PEG - Safety Data Sheet

Safety Data Sheet (DEU) HDAC4 p-S246 Kit / 64HDAC4S6PEH