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HTRF Human Apolipoprotein E Kit HTRF®

The ApoE kit is designed for the simple quantification of ApoE in cell-based formats.
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  • Highly accurate Highly accurate
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
  • Ready-to-use Ready-to-use
The ApoE kit is designed for the simple quantification of ApoE in cell-based formats.
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Overview

Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are key in maintaining the structural integrity and solubility of lipoproteins. ApoE is a fat-binding protein that is part of the chylomicron and Intermediate-Density Lipoprotein (IDLs). It is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. A correlation between ApoE and neurodegenerative conditions such as multiple sclerosis and Alzheimer's disease has been found.

Benefits

  • RESULTS IN LESS THAN 3 HOURS
  • PLASMA & CELL COMPATIBLE

Assay principle

The Apolipoprotein E assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.
ApoE kit principle

Assay protocol

The ApoE assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 3-hour incubation period at RT.
ApoE kit protocol

Apolipoprotein E standard curve

10 µL of recombinant protein in diluent #5 were dispensed to a low volume white microplate before the addition of 10 µL of the premixed HTRF anti ApoE detection reagents. The HTRF signal was recorded after 2h incubation at room temperature.

Human ApoE standard curve

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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