Human Apolipoprotein C3 kit
ApoC3 quantification in cells, supernatants and plasma
Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are key in maintaining the structural integrity and solubility of lipoproteins. ApoE is a fat-binding protein that is part of the chylomicron and Intermediate-Density Lipoprotein (IDLs). It is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. A correlation between ApoE and neurodegenerative conditions such as multiple sclerosis and Alzheimer's disease has been found.
10 µL of recombinant protein in diluent #5 were dispensed to a low volume white microplate before the addition of 10 µL of the premixed HTRF anti ApoE detection reagents. The HTRF signal was recorded after 2h incubation at room temperature.
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