HTRF Human Total ADAR1 Detection Kit HTRF®

Associated products

The HTRF Human ADAR1 Detection Kit is designed to monitor the expression level of cellular ADAR1.

All inclusive kit
Low sample consumption
No-wash
High sensitivity

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The HTRF Human ADAR1 Detection Kit is designed to monitor the expression level of cellular ADAR1.

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Overview

ADAR1 is one of two ADAR gene-coded enzymes. It binds to double-stranded RNA (dsRNA) and deaminates its adenosine into inosine (hypoxanthine). ADAR proteins act post-transcriptionally to alter RNA nucleotide contents. The deamination they operate interferes with the usual A:U pairing and destabilizes the RNA.

The loss of regulation of ADAR1 & ADAR2 is involved in the development and progression of multiple cancers, such as glioblastomas, melanomas, or acute leukemias. On top of their role in oncogenesis, ADAR enzymes are suspected of contributing to the aggravation of some infectious diseases like HIV and measles, but also of mental disorders such as depression, epilepsy, and schizophrenia.

Benefits

SPECIFICITY
PRECISION
LOW SAMPLE CONSUMPTION

Validation of Total ADAR1 detection in siRNA-treated HepG2 cells

HepG2 cells were plated at different cell densities under 80µl in a 96-well plate in complete culture medium, and incubated overnight at 37°C, 5% CO2.

After culture, the cells were treated with siRNA by adding 20µl of a mix of Lipofectamine® RNAiMax/siRNA for ADAR1, then incubated for 24h and 48h at 37°C, 5% CO2. For untreated cells, 20µl of OptMEM medium were added instead of the siRNA mix.

After incubation, cells were lysed with 50µL of supplemented 1X lysis buffer #1 for 30 minutes at RT under gentle shaking, and 16 µL of lysate were transferred into a low volume white microplate before adding 2µL of the HTRF d2 detection reagent and 2µL HTRF Eu-K detection reagent. The HTRF signal was recorded after an ON incubation.

Validation of Total ADAR1 detection in siRNA-treated HepG2 cells

HTRF cellular phospho-protein assays

Physiologically relevant results fo fast flowing research - Flyers

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Insider Tips for successful sample treatment - Technical Notes

Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Technical Notes

Best practices for analyzing tumor xenografts with HTRF phospho assays

Protocol for tumor xenograft analysis with HTRF - Technical Notes

Key guidelines to successful cell signaling experiments

Mastering the art of cell signaling assays optimization - Guides

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Detailed protocol and direct comparison with WB - Posters

Implement HTRF phospho-protein assays at every step of the drug discovery process, from in vitro to in vivo models

A single technology for 2D cells, 3D cells, and xenograft models - Posters

Simplify complex pathway dissection by combining the power of HTRF® cellular phospho-assays and the flexibility of the CyBi®-FeliX liquid handling system

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Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery

One technology across all samples - Application Notes

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

Tumor xenograft analysis: HTRF versus Western blot - Application Notes

HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Application Notes

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Application Notes

HTRF Alpha-tubulin Housekeeping kit

Properly interpret your compound effect - Application Notes

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Application Notes

How to run a cell based phospho HTRF assay

What to expect at the bench - Videos

Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening

In collaboration with Bayer - Scientific Presentations

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Videos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Videos

Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection

Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes

Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)

Learn how to reduce time and sample consumption - Application Notes

Assessment of drug efficacy and toxicity by combining innovative technologies

Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes

Product Insert ATG14 Total Kit / 64ATG14TPEG-64ATG14TPEH

64ATG14TPEG-64ATG14TPEH - Product Insert

Product Insert ADAR1 Total Kit / 64ADAR1TPEG-64ADAR1TPEH

64ADAR1TPEG-64ADAR1TPEH - Product Insert

Safety Data Sheet (DEU) ADAR1 Total Kit / 64ADAR1TPEG