Phospho-STAT3 (Tyr705) cellular kit
All-in-one kit for robust detection of Phospho-STAT3 (Ser423/425)
HTRF Human Total MET Detection Kit HTRF®
The HTRF Total MET detection kit is designed to monitor the expression level of cellular MET.
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All inclusive kit
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Low sample consumption
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No-wash
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High sensitivity
Overview
c-MET, also called tyrosine-protein kinase MET or hepatocyte growth factor receptor (HGFR), is a human MET gene-coded protein with tyrosine kinase activity. It acts as a tyrosine kinase receptor, with functions related to wound healing, organogenesis, and embryonic development.
MET is expressed in epithelial cells and is triggered by hepatocyte factors HGF and HSF, with ramifications into various pathways like RAS-MAPK, AKT, and STAT3 signaling.
Abnormal activation of MET is linked to cancer progression, with effects on tumor growth, angiogenesis, and tumor cell migration & invasion. These relationships between MET and tumor cells are found across multiple cancer types (liver, brain, breast, kidney, etc) and are suspected to originate from a hijacking of MET and its ligand expression regulation, which leads to their overexpression and autocrine activation of MET.
Benefits
- SPECIFICITY
- PRECISION
- LOW SAMPLE CONSUMPTION
Validation of Phospho & Total MET detection in HGF-stimulated A431 cells
A431 cells were plated under 10µL in a 96-well plate (100,000 cells/well) in complete culture medium, and incubated overnight at 37ºC, 5% CO2. The next day, medium was removed and 50µL of complete medium was added before a 2h incubation at 37ºC, 5% CO2. After the incubation, the cells were treated with 50µL of increasing concentrations of human HGF diluted in complete medium for 15 minutes at 37ºC, 5% CO2.
After medium removal, cells were then lysed with 60 µL of lysis buffer #2 and phospho-total protein blocking reagent for 45 minutes at RT under gentle shaking. After lysis, 16µL of lysate were transferred into a low volume white microplate before the addition of 2µL each of the donor and acceptor HTRF Phospho-MET (Y1234/1235) or Total MET detection reagents. The HTRF signal was recorded after an overnight incubation.
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Product Insert ATG14 Total Kit / 64ATG14TPEG-64ATG14TPEH
64ATG14TPEG-64ATG14TPEH - Product Insert
Product Insert MET Total Kit / 64METTPEG-64METTPEH
64METTPEG-64METTPEH - Product Insert
Safety Data Sheet (ELL) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (ITA) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (SPA) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (DEU) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) MET Total Kit / 64METTPEG
64METTPEG - Safety Data Sheet
Safety Data Sheet (DEU) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Safety Data Sheet (ELL) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Safety Data Sheet (ITA) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Safety Data Sheet (SPA) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) MET Total Kit / 64METTPEH
64METTPEH - Safety Data Sheet
Plate Reader Requirement
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