Human Apolipoprotein B kit

The ApoB kit is designed for the simple quantification of ApoB in cell-based formats.
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  • Ready-to-use
  • Highly accurate
  • Faster and more convenient than ELISA
The ApoB kit is designed for the simple quantification of ApoB in cell-based formats.
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In stock

Overview

Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are key in maintaining the structural integrity and solubility of lipoproteins, and play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. ApoB is the main apolipoprotein of chylomicrons and low-density lipoproteins (LDLs). ApoB and the ApoB/ApoA1 ratio are thought to be an ideal marker of vascular disease risks.

Assay principle

The Apolipoprotein B assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.

Assay Protocol

The ApoB assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 3-hour incubation period at RT.

Key features

Cross-reactivity (%)
ApoB
ApoA10.004
ApoB-100100
ApoA2<0.001
ApoC1<0.001
ApoC20.001
ApoC30.006
ApoE0.004

ApoB detection upon Retinoid X receptor (RXR) agonist treatment

11885, low glucose) supplemented with 2% FBS. 5 µL of HepG2 cells (2,000 & 5,000 per well) and 5 µL of the RXR ligand 9-cis retinoic acid (9-cis-RA) in DMEM, 2% FBS at various concentrations were dispensed into a 384-well low volume black culture plate. After a 48 hour incubation at 37°C in CO2 atmosphere, 10 µL of the two pre-mixed detection reagents were added.'

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