Human FMRP kit
Human FMRP quantification in cell lysates
Human Apolipoprotein B kit HTRF®
The ApoB kit is designed for the simple quantification of ApoB in cell-based formats.
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Ready-to-use
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Highly accurate
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Faster and more convenient than ELISA
Overview
Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are key in maintaining the structural integrity and solubility of lipoproteins, and play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. ApoB is the main apolipoprotein of chylomicrons and low-density lipoproteins (LDLs). ApoB and the ApoB/ApoA1 ratio are thought to be an ideal marker of vascular disease risks.
Benefits
- RESULTS IN LESS THAN 3 HOURS
- PLASMA & CELL COMPATIBLE
Assay principle
The Apolipoprotein B assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.
Assay Protocol
The ApoB assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure.
This protocol requires a single 3-hour incubation period at RT.
Key features
Cross-reactivity (%) | ApoB |
| ApoA1 | 0.004 |
| ApoB-100 | 100 |
| ApoA2 | <0.001 |
| ApoC1 | <0.001 |
| ApoC2 | 0.001 |
| ApoC3 | 0.006 |
| ApoE | 0.004 |
ApoB detection upon Retinoid X receptor (RXR) agonist treatment
11885, low glucose) supplemented with 2% FBS. 5 µL of HepG2 cells (2,000 & 5,000 per well) and 5 µL of the RXR ligand 9-cis retinoic acid (9-cis-RA) in DMEM, 2% FBS at various concentrations were dispensed into a 384-well low volume black culture plate. After a 48 hour incubation at 37°C in CO2 atmosphere, 10 µL of the two pre-mixed detection reagents were added.'
Product Insert APO B (h) Kit / 64APBPEG
64APBPEG - Product Insert
Product Insert APO B (h) Kit / 64APBPEH
64APBPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Cardiovascular Research With HTRF Assays
Learn how peer researchers tackle cardiovascular research with HTRF assays - Application Notes
Safety Data Sheet (DEU) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (ELL) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (ITA) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (SPA) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) APO B (h) Kit / 64APBPEG
64APBPEG - Safety Data Sheet
Safety Data Sheet (DEU) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Safety Data Sheet (ELL) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Safety Data Sheet (ITA) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Safety Data Sheet (SPA) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) APO B (h) Kit / 64APBPEH
64APBPEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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