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Human Apolipoprotein C3 kit HTRF®

The ApoC3 kit is designed for the simple quantification of ApoC3 in cell-based formats.

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  • Ready-to-use Ready-to-use
  • Highly accurate Highly accurate
  • Faster and more convenient than ELISA Faster and more convenient than ELISA

The ApoC3 kit is designed for the simple quantification of ApoC3 in cell-based formats.

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Overview

Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are important in maintaining the structural integrity and solubility of lipoproteins, and play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. ApoC3 inhibits lipoprotein lipase and hepatic lipase. Increased ApoC3 expression may lead to hypertriglyceridaemia and an atherogenic lipoprotein profile.

Benefits

  • RESULTS IN LESS THAN 3 HOURS
  • PLASMA & CELL COMPATIBLE

Assay principle

The Apolipoprotein C3 assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.
ApoC3 kit principle

Assay Protocol

The ApoC3 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 2-hour incubation period at RT.
ApoC3 kit protocol

Key features


Human Apo CIII
Incubation2h at RT (or ON)
Assay range0.321 to 125 ng/mL
Detection limit0.112 ng/mL

Intra/Inter assay

Intra-assay (n=22)

Sample

Mean [ApoC3] (ng/mL)

CV

1

1.15

13.2

2

11.8

9

3

121.8

9.5


Mean CV

10.5

Each of the 3 samples was measured 22 times, and the %CV for the concentration was calculated for each sample

Inter-assay (n=4)

Sample

[ApoC3] (ng/mL)

Mean (delta R)

CV

1

9.792986

8

2

50.612644

11

3

105

21141

7



Mean CV

8.6

Each of the samples was measured in 4 different experiments, and the %CV for the concentration was calculated for each sample.

Product Insert APO C3 (h) Kit / 63ADK001PEG-63ADK001PEH

63ADK001PEG-63ADK001PEH - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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