Human CCL2 (MCP1) kit HTRF®

Name: Human CCL2 (MCP1) kit
Brand: Cisbio
SKU: 40392
Availability: InStock

Associated products

The HTRF human CCL2 (MCP1) kit is designed for the quantification of human CCL2 release in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

Download the protocols

The HTRF human CCL2 (MCP1) kit is designed for the quantification of human CCL2 release in cell supernatant.

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Overview

Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages, and dendritic cells to attract monocytes, basophils, and T cells. CCL2 is involved in pathologies like psoriasis or atherosclerosis. In the brain, CCL2 is released by neurons, astrocytes, and microglia, and is part of the neuro-inflammatory response observed in ischemia or Alzheimer's disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to diabetes.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human CCL2/MCP1 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SCALABLE FROM 96 TO 1536WELLS
PANEL QUANTIFICATION

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL2 analysis.

Technical specifications of human CCL2 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent)	20 pg/mL & 90 pg/mL
Range	90 - 3,000pg/mL
Time to result	ON at RT
Calibration	NIBSC (92/794) value (IU/mL) = 0,9 x HTRF hCCL2 value (pg/mL)
LOD & LOQ (in Diluent)	20 pg/mL & 90 pg/mL
Species	Human only

Intra and inter assay

Sample	Mean [CCL2] (pg/mL)	CV
1	100	10%
2	1500	7%
3	2750	7%
	Mean CV	8%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter assay (n=4)

Sample	[CCL2] (pg/mL)	Mean (delta R)	CV
1	121	335	2%
2	437	1096	3%
3	1579	3030	2%
		Mean CV	2%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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