Active GLP-1 kit
Active GLP1 quantification in cell supernatants
Human CCL20 (MIP3 alpha) kit HTRF®
The HTRF human CCL20 (MIP3 alpha) kit is designed for the quantification of human CCL20 release in cell supernatant.
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No-wash
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Low sample consumption
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Under 2h bench time
Overview
Also called LARC (liver activation regulated chemokine) or MIP3A (macrophage inflammatory protein 3), CCL20 is a chemokine mainly present in peripheral blood lymphocytes, lymph nodes, and the liver. CCL20 secretion is upregulated by microbial factors and inflammatory cytokines like TNFa or IFNg. Its main role is to attract lymphocytes and dendritic cells to the site of infection. In the CNS, CCL20 released by endothelial cells has been shown to attract TH17 cells and promote their infiltration to the lumbar spinal cord.
Benefits
- SCALABLE FROM 96 TO 1536WELLS
- PANEL QUANTIFICATION
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up toa1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL20 analysis.
Technical specifications of human CCL20 kit
| Sample size | 16 µL |
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 15 pg/mL &47 pg/mL |
| Range | 47 – 2,000 pg/mL |
| Time to result | ON at RT |
| Species | Human only |
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [CCL20] (pg/mL) | CV |
|---|---|---|
| 1 | 250 | 8% |
| 2 | 750 | 5% |
| 3 | 1500 | 4% |
| Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [CCL20] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 263 | 503 | 9% |
| 2 | 593 | 1124 | 8% |
| 3 | 1333 | 2250 | 11% |
| Mean CV | 9% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF solutions for each cell type - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert CCL20 (h) Kit / 62HCC20PEG-62HCC20PEH
62HCC20PEG-62HCC20PEH - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (ELL) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (ITA) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (SPA) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) CCL20 (h) Kit / 62HCC20PEG
62HCC20PEG - Safety Data Sheet
Safety Data Sheet (DEU) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Safety Data Sheet (ELL) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Safety Data Sheet (ITA) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Safety Data Sheet (SPA) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) CCL20 (h) Kit / 62HCC20PEH
62HCC20PEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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