Human CCL20 (MIP3 alpha) kit HTRF®

Name: Human CCL20 (MIP3 alpha) kit
Brand: Cisbio
SKU: 40390
Availability: InStock

Associated products

The HTRF human CCL20 (MIP3 alpha) kit is designed for the quantification of human CCL20 release in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

Download the protocols

The HTRF human CCL20 (MIP3 alpha) kit is designed for the quantification of human CCL20 release in cell supernatant.

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Overview

Also called LARC (liver activation regulated chemokine) or MIP3A (macrophage inflammatory protein 3), CCL20 is a chemokine mainly present in peripheral blood lymphocytes, lymph nodes, and the liver. CCL20 secretion is upregulated by microbial factors and inflammatory cytokines like TNFa or IFNg. Its main role is to attract lymphocytes and dendritic cells to the site of infection. In the CNS, CCL20 released by endothelial cells has been shown to attract TH17 cells and promote their infiltration to the lumbar spinal cord.

Benefits

SCALABLE FROM 96 TO 1536WELLS
PANEL QUANTIFICATION

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up toa1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL20 analysis.

Technical specifications of human CCL20 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	15 pg/mL &47 pg/mL
Range	47 – 2,000 pg/mL
Time to result	ON at RT
Species	Human only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [CCL20] (pg/mL)	CV
1	250	8%
2	750	5%
3	1500	4%
	Mean CV	6%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[CCL20] (pg/mL)	Mean (delta R)	CV
1	263	503	9%
2	593	1124	8%
3	1333	2250	11%
		Mean CV	9%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Can HTRF technology answer to different scientific questions? An academic with industrial standards point of view

In collaboration with CNRS - Scientific Presentations

HTRF Cytokine cell-based assays

Monitor immune regulation the simple way - Flyers

Inflammation cell by cell

HTRF solutions for each cell type - Flyers

HTRF assays for Oncology and Inflammation

Signaling in the Immune System - Brochures

HTRF measurement of cytokine release from fresh blood samples

In collaboration with Blood assay solution - Application Notes

Cytokine secretion profile on PBMCs

One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Guideline for PBMC freezing - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

Product Insert CCL20 (h) Kit / 62HCC20PEG-62HCC20PEH

62HCC20PEG-62HCC20PEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Cytokine assays: a guide to success with HTRF

Setting up a successful cytokine assay - Guides

Portfolio of Life Sciences Cytokine Assays

This leaflet presents the analytical performances of each assay - Brochures

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Safety Data Sheet (DEU) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (ELL) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (ITA) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (SPA) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (ENG-US) CCL20 (h) Kit / 62HCC20PEG

62HCC20PEG - Safety Data Sheet

Safety Data Sheet (DEU) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (ELL) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (FRA-FR) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (ITA) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (SPA) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (ENG-GB) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (ENG-US) CCL20 (h) Kit / 62HCC20PEH

62HCC20PEH - Safety Data Sheet

Safety Data Sheet (DEU) CCL20 (h) Kit / 62HCC20PEJ