Human G-CSF kit
No-wash kit to quantify released Human G-CSF
The HTRF human CCL3 (MIP1 alpha) kit is designed for the quantification of human CCL3 release in cell supernatant.
Also called MIP-1 alpha (macrophage inflammatory protein 1 alpha), CCL3 is a member of the MIP (macrophage inflammatory protein) family, which also includes CCL4 (MIP1 beta). This chemokine is secreted by monocytes, macrophages, T cells, and endothelial cells. CCL3 plays an important role in the activation of the inflammatory response by attracting monocyte-lineae cells and lymphocytes to inflammation sites. CCL3 has been reported as a stem cell inhibitory factor.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Humant CCL3/MIP-1α AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL3 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol|
|LOD &LOQ (in diluent)||28 pg/mL &58 pg/mL|
|Range||58 4,400 pg/mL|
|Time to result||ON @ RT|
|Calibration||NIBSC (92/518) value (IU/mL) = 0.00005 x HTRF hCCL3 value (pg/mL)|
intra assay (n=24)
|Sample||Mean [CCL3] (pg/ml)||cv|
inter assay (n=4)
|Sample||[CCL3] (pg/ml)||Mean (delta ratio)||Cv|
In collaboration with CNRS - Scientific Presentations
In collaboration with Molecular Devices - Application Notes
In collaboration with Blood assay solution - Application Notes
Perform and optimize cytokine assays on PBMC - Technical Notes
A fun video introducing you to cytokines assays with HTRF - Videos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures