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Human CTGF Kit HTRF®

Associated products
  • Photography of Hu-CTGF kit
  • Photography of Hu-CTGF kit

The HTRF human CTGF kit is designed for the accurate measurement of human CTGF released into cell supernatants.

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  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
  • Highly accurate Highly accurate
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The HTRF human CTGF kit is designed for the accurate measurement of human CTGF released into cell supernatants.

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Overview

Connective tissue growth factor CTGF, also known as CCN2, is a matricellular signaling modulator.

CTGF regulates a range of cellular functions, including cell adhesion, aggregation, and migration. CTGF modulates the balance of tissue remodeling in a large variety of cell types, such as vascular endothelial cells, fibroblasts, epithelial cells, and smooth muscle.

CTGF is primarily regulated at the level of transcription and is induced by mechanical stresses and a number of cytokines and growth factors, including TGFβ.

The HTRF human CTGF kit is a homogeneous add and read assay, to measure CTGF secretion in cell supernatants.

Benefits

  • Validated on human MRC5 and IMR90 cell lines.

Intra / Inter assay

Intra-assay (n=24)

Sample

Mean [CTGF] (ng/mL)

CV

1

10.1

14.7

2

69.25

10.6

3

475

10


Mean CV

11.7

Each of the 3 samples was measured 24 times, and the %CV for the concentration was calculated for each sample

Inter-assay (n=4)

Sample

[CTGF] (ng/mL)

Mean (delta R)

CV

1

19.2

1100

5

2

131.6

14922

11

3

250

24765

2



Mean CV

6

Each of the samples was measured in 4 different experiments, and the %CV for the concentration was calculated for each sample.

Spike and recovery

Sample

[CTGF] recomb prot (ng/mL)

[CTGF] native prot (ng/mL)



[CTGF]expected (pg/mL)

[CTGF]detected (pg/mL)

Recovery

1

14.02

15.02



29.04

34.73

120 %

2

14.02

22.05



36.07

39.1

108%

3

25.19

16.88



42.07

42.07

110%

Different amounts of recombinant CTGF were added to MEM + 1% BSA cell supernatant samples containing a known concentration of CTGF . Samples were mixed at a 1:1 volume ratio. The set of responses obtained from a standard curve was compared to the calculated expected values. The recovery value observed validates the sample matrix used for this assay.

CTGF secretion in MRC5 cell line stimulated with TGFbeta

Human MRC5 cells were plated at 400,000 cells/well in a 12-well plate and cultured overnight with 600 µL of complete MEM cell culture medium, at 37°C, 5% CO2. Cells were then stimulated with increasing concentrations of TGFbeta (0.2 to 20 ng/mL) under 600 µL of MEM + 1%. After 24h of stimulation, 16 µL of supernatant were transferred into a white detection plate to be analyzed using the Human CTGF assay. The HTRF signal was recorded after an overnight incubation.

Validation of Human CTGF kit on human MRC5 cell lines
View product citations for Biomarkers 64CTGFPEG on CiteAb

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Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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