Human Fc kit
- Highly specific
- Faster and more convenient than ELISA
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human Fc kit enables a rapid quantification of human IgG, and any proteins containing a human Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
|Detection limit:||5.5 ng/mL|
|Dynamic range:||5.5 to 4,000 ng/mL|
|Incubation time:||2h30 to ON|
Figure 1: Various concentrations of four different human Fc chimeras were tested using the Total human IgG assay, following standard assay protocols.
Figure 2 demonstrates the compatibility of the HTRF human Fc assay with different media, even in the presence of 10% FCS. No impact on assay performance was seen. Unlike other immunoassays involving streptavidin-biotin detection, this assay is insensitive to the presence of culture medium, whose quenching effects can be compensated for by our patented 665/620 ratio.
Figure 2: IgG standard curve was drawn up using IgG in phosphate buffer, culture media and RPMI containing 10% FCS+/- 4.4% PSGHT (Penicillin, Streptamycin, Glutamine, Hypoxanthine, Thymidine).
In collaboration with MRC - Scientific Presentations
HTRf® reagents for biotherapeutics - Flyers
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