-->

Human FMRP kit HTRF®

The Human FMRP kit is designed for the rapid detection of human FMRP in cell lysates.
See more
  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Human FMRP kit is designed for the rapid detection of human FMRP in cell lysates.
-

Overview

FMRP (Fragile X Mental Retardation Protein) is a brain protein known to play an important role in normal cognitive development and female reproductive function. fmr1 gene silencing has been correlated to fragile X syndrome onset triggering severe learning deficits or mental retardation. Mutations of the fmr1 gene can also be associated to intellectual disability, premature ovarian failure, autism, Parkinson's disease, developmental delays, and other cognitive deficits. The human FMRP kit enables the fast monitoring of FMRP produced by cells.

Benefits

  • RESULTS IN LESS THAN 2 HOURS
  • FMR1 GENE EXPRESSION STUDY

Assay principle

Human FMRP is measured using a sandwich immunoassay involving two specific anti-FMRP antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the FMRP present in the sample.
FMRP kit principle

Assay Protocol

The human FMRP assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Human FMRP. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
FMRP kit protocol

Product Insert FMRP (h) Kit / 63ADK038PEG-63ADK038PEH

63ADK038PEG-63ADK038PEH - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

Let's find your reader