Human Frataxin HTRF®

This HTRF kit is designed for the rapid detection of human Frataxin in cell lysates.

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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA

This HTRF kit is designed for the rapid detection of human Frataxin in cell lysates.



Frataxin is a protein localized in the mitochondrion that acts either as an iron chaperone or an iron storage protein. Frataxin mRNA is predominantly expressed in tissues with a high metabolic rate such as the liver, kidneys, adipose tissue, and heart. Reduced expression of Frataxin is the cause of Friedreich's ataxia (FRDA), a lethal neurodegenerative disease. The human Frataxin kit enables the fast monitoring of Frataxin produced by cells.



Assay principle

Human Frataxin is measured using a sandwich immunoassay involving two specific Frataxin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of Frataxin present in the sample.
Frataxin kit principle

Assay Protocol

The human Frataxin assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Human Frataxin. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Frataxin kit protocol

Product Insert Frataxin (h) Kit / 63ADK039PEG-63ADK039PEH

63ADK039PEG-63ADK039PEH - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Molecular basis of neuroinflammation and neurodegeneration diseases

The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides

Neurodegeneration and its main related diseases

Discover this infographic design on neurodegenerative diseases - Infographics

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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