Human Heme Oxygenase kit

The Human Heme Oxygenase kit is designed for the rapid detection of HO-1 in cell lysates.
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  • Ready-to-use
  • Highly specific
  • Faster and more convenient than ELISA
The Human Heme Oxygenase kit is designed for the rapid detection of HO-1 in cell lysates.
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In stock

Overview

The human Heme Oxygenase 1 (HO-1) is an enzyme that catalyzes the degradation of Heme leading to the production of biliverdin, ferrous iron, and carbon monoxide. HO-1 is an inducible isoform in response to stress such as oxidative stress, hypoxia, heavy metals, cytokines, etc. The human Heme Oxygenase 1 kit enables the fast monitoring of HO-1 produced by cells.

Assay principle

Human heme oxygenase is measured using a sandwich immunoassay involving two specific human heme oxygenase antibodies, respectively labelled with Europium Cryptate (donor) and d2 (Acceptor). The intensity of the signal is proportional to the concentration of heme oxygenase present in the sample.

Assay Protocol

The human heme oxygenase 1 assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Human HO-1. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Product Insert Heme Oxy (h) Kit / 63ADK044PEG-63ADK044PEH

63ADK044PEG-63ADK044PEH - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

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