Human IFN alpha kit HTRF®

Name: Human IFN alpha kit
Brand: Cisbio
SKU: 40409
Availability: InStock

Associated products

The HTRF human IFN alpha kit is designed for the quantification of human IFN alpha release in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

Download the protocols

The HTRF human IFN alpha kit is designed for the quantification of human IFN alpha release in cell supernatant.

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Overview

Mainly produced by leukocytes, IFN alpha is involved in innate immunity response to viral infection. IFN alpha activates several intracellular pathways in target cells, which explains its pleiotropic effects on cell differentiation, growth, and immunoregulation.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IFN-α AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SCALABLE FROM 96 TO 1536WELLS
PANEL QUANTIFICATION

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IFN alpha analysis.

Technical specifications of human IFN alpha kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	11 pg/mL &36 pg/mL
Calibration	NIBSC (94/784) value (IU/mL) = 0,9 x HTRF hIFNa HTRF
Range	36 4,000 pg/mL
Time to result	2h at RT
Species	Human only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IFNa] (pg/mL)	CV
1	150	11%
2	1500	2%
3	3000	5%
	Mean CV	6%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IFNa] (pg/mL)	Mean (delta R)	CV
1	47	268	9%
2	206	1150	5%
3	1905	4227	4%
		Mean CV	6%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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