Human Fc kit
Human Fc quantification for bioprocess applications
Human IgG Kappa MAb kit HTRF®
The Human IgG Kappa MAb kit is designed for the fast quantification of human IgGs Kappa light chains.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG Kappa MAb kit enables a rapid quantification of human IgG Kappa light chains in cell supernatant. The assay is compatible with natural IgG as well as recombinant antibodies.
Benefits
- BIOPROCESS QUALITY CONTROL
- SPECIFIC TO IgG1, 2, 3, AND 4
Assay principle
The human IgG Kappa MAb kit is detected in a sandwich assay format using 2 different specific antibodies. The anti-(h)-IgG Kappa antibody is labelled with d2 (acceptor) and the anti(h)-Fc antibody is labelled with Eu3+-Cryptate (donor). The two antibodies bind to the (h)IgG Kappa present in the sample, thereby generating FRET. The specific signal modulates positively in proportion to (h)IgG Kappa.
Assay Protocol
The human IgG Kappa MAb assay features a streamlined protocol with only 1 incubation step after sample and IgG Kappa MAb detection reagents dispensing.
This protocol requires a single two-hour incubation period at RT.
Key features
| Detection limit | 0.8 ng/mL |
| Dynamic range | 0.8 to 100 ng/mL |
| Specificity | subclasses IgG1, IgG2, IgG3 & IgG4 |
| Cross reactivity | 0% Human Lambda |
| 0% Mouse Kappa | |
| <1% Human IgM |
Accelerate throughput without compromising on performance
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Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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