Human Fc kit
Human Fc quantification for bioprocess applications
Human IgG kit HTRF®
The Human IgG kit is designed for the fast quantification of human IgG.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG kit enables a rapid quantification of human IgG in cell supernatant and the selection of the best producer clones. The assay is compatible with natural IgG as well as recombinant antibodies.
Benefits
- BIOPROCESS QUALITY CONTROL
- HIGH PRODUCING CLONE SELECTION
Assay principle
This kit is a sandwich immunoassay enabling highly sensitive quantification of human IgG, even when using a small sample volume. The assay uses two polyclonal antibodies, one (Fc specific) labelled with Cryptate and the second (FAb specific) with d2. The signal intensity is proportional to the number of antibody-antibody complexes formed and therefore to the human IgG concentration.
Assay Protocol
The human IgG assay features a streamlined protocol with only 1 incubation step after sample and human IgG detection reagents dispensing.
This protocol requires a single two-hour incubation period at RT.
Key features
The figure below shows the human IgG kit calibration curve. The assay was performed in a 384-well small volume white microplate in a 20 µL total volume, using 10µL human IgG calibrator diluted in PBS 0.1 M and 5 µL of each conjugate. The two HTRF conjugates may be pre-mixed and added in a single dispensing step to further streamline the protocol. After 2h of incubation, the signal was read on a PHERAstarFS flash lamp.
| Detection limit | 0.9 ng/mL |
|---|---|
| Assay range | 0.9 to 2000 ng/mL |
| Incubation time | 2h at RT |
| Specificity | IgG1, IgG2, IgG3, IgG4 |
Accelerate throughput without compromising on performance
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A guide to Homogeneous Time Resolved Fluorescence
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How HTRF compares to Western Blot and ELISA
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Safety Data Sheet (ITA) IgG (h) Kit / 6FHIGPEJ
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Safety Data Sheet (SPA) IgG (h) Kit / 6FHIGPEJ
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Safety Data Sheet (ENG-GB) IgG (h) Kit / 6FHIGPEJ
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Safety Data Sheet (ENG-US) IgG (h) Kit / 6FHIGPEJ
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Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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