Human IL10 kit HTRF®

Name: Human IL10 kit
Brand: Cisbio
SKU: 40423
Availability: InStock

Associated products

The HTRF human IL10 kit is designed for the quantification of human IL10 release in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

Download the protocols

The HTRF human IL10 kit is designed for the quantification of human IL10 release in cell supernatant.

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Overview

Also known as CSIF (Cytokine synthesis inhibitory factor), IL10 is an anti-inflammatory cytokine. Its negative regulation of the immune response is due to its ability to block the signaling of other cytokines, such as IFN gamma, and to inhibit CD28 phosphorylation in T cells. In immuno-oncology, IL10 is known to inhibit the expansion of TH1 cells while promoting the development of regulatory T cells (Treg) and macrophages.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-10 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SCALABLE FROM 96 TO 1536WELLS
REGULATORY CELLS FUNCTIONAL READOUT

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL10 analysis.

Technical specifications of human IL10 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	7 pg/ml &22 pg/mL
Range	22 6,000 pg/mL
Calibration	NIBSC (93/722) value (IU/mL) = 0,005 x HTRF hIL10 value (pg/mL)
Time to result	Overnight at RT
Species	Human only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IL10] (pg/mL)	CV
1	62	9%
2	3302	2%
3	5725	3%
	Mean CV	5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IL10] (pg/mL)	Mean (delta R)	CV
1	93	717	14%
2	493	4641	11%
3	2609	32182	11%
		Mean CV	12%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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In collaboration with CNRS - Scientific Presentations

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Perform and optimize cytokine assays on PBMC - Technical Notes

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Guideline for PBMC freezing - Technical Notes

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A fun video introducing you to cytokines assays with HTRF - Videos

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Get the brochure about technology comparison. - Brochures

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