Human IL2 kit
No-wash kit to quantify released Human IL2
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The HTRF human IL10 kit is designed for the quantification of human IL10 release in cell supernatant.
Also known as CSIF (Cytokine synthesis inhibitory factor), IL10 is an anti-inflammatory cytokine. Its negative regulation of the immune response is due to its ability to block the signaling of other cytokines, such as IFN gamma, and to inhibit CD28 phosphorylation in T cells. In immuno-oncology, IL10 is known to inhibit the expansion of TH1 cells while promoting the development of regulatory T cells (Treg) and macrophages.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-10 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL10 analysis.
Sample size | 16 µL |
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 7 pg/ml &22 pg/mL |
Range | 22 6,000 pg/mL |
Calibration | NIBSC (93/722) value (IU/mL) = 0,005 x HTRF hIL10 value (pg/mL) |
Time to result | Overnight at RT |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL10] (pg/mL) | CV |
---|---|---|
1 | 62 | 9% |
2 | 3302 | 2% |
3 | 5725 | 3% |
Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL10] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 93 | 717 | 14% |
2 | 493 | 4641 | 11% |
3 | 2609 | 32182 | 11% |
Mean CV | 12% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF solutions for each cell type - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert IL10 (h) Kit / 62HIL10PEG-62HIL10PEH
62HIL10PEG-62HIL10PEH - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
Novel HTRF platform to delineate STING pathway
Explore the whole STING pathway with a single technology - Posters
Discover no-wash cytokine assay solutions - Flyers
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL10 (h) Kit / 62HIL10PEG
62HIL10PEG - Safety Data Sheet
Quality Control Report IL10 (h) Kit / 20241117
62HIL10PEG Batch 06E - Quality Control Report
Safety Data Sheet (DEU) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL10 (h) Kit / 62HIL10PEH
62HIL10PEH - Safety Data Sheet
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