Human IL1 alpha kit
No-wash kit to quantify released Human IL1 alpha
Human IL18 kit HTRF®
The HTRF human IL18 kit is designed for the quantification of human IL18 release in cell supernatant.
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No-wash
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Low sample consumption
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Under 2h bench time
Overview
Also called IGIF (interferon gamma inducing factor), IL18 is a pro-inflammatory cytokine secreted mainly by macrophages. Its biological activity is close to that of IL12, and it can enhance NK cell activity as well as induce IFNg secretion by PBMC. An important role of IL18 is eliminating VEGF from the eye, thus protecting the retina. It is used together with anti-VEGF antibodies in the treatment of AMD (age-related macular degeneration). In addition to this physiological role, IL18 dysregulation leads to severe inflammatory reactions.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-18 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
Benefits
- SCALABLE FROM 96 TO 1536WELLS
- PANEL QUANTIFICATION
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL18 analysis.
Technical specifications of human IL18 kit
| Sample size | 16 µL |
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 18 pg/mL &43 pg/mL |
| Range | 43 8,000 pg/mL |
| Time to result | 1h at RT |
| Calibration | NIBSC (03/200) value (IU/mL) = 5 x HTRF hIL18 value (pg/mL) |
| Species | Human only |
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL18] (pg/mL) | CV |
|---|---|---|
| 1 | 100 | 10% |
| 2 | 4500 | 3% |
| 3 | 6800 | 4% |
| Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL18] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 155 | 410 | 3% |
| 2 | 751 | 1855 | 5% |
| 3 | 3636 | 6570 | 5% |
| Mean CV | 4% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert IL18 (h) Kit / 62HIL18PEG-62HIL18PEH
62HIL18PEG-62HIL18PEH - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
HTRF HMGB1, IL-1β and IL-18 assays discriminate between inflammasome triggered pyroptosis, and necroptosis - Application Notes
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL18 (h) Kit / 62HIL18PEG
62HIL18PEG - Safety Data Sheet
Safety Data Sheet (DEU) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL18 (h) Kit / 62HIL18PEH
62HIL18PEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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