Human IFN gamma kit
No-wash kit to quantify released Human IFN gamma
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The HTRF human IL2 kit is designed for the quantification of human IL2 release in cell supernatant.
IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes the differentiation of T cells into effector or memory T cells, thus helping the body fight off infection. IL2 is also a key player in immuno-oncology since it is the main promoter of the differentiation of T cells into Treg (regulatory T cells).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-2 AlphaLISA kitand the Human IL-2 AlphaLISA biotin-free kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL2 analysis.
Sample size | 16 µL |
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 7 pg/mL &37 pg/mL |
Range | 37 8,000 pg/mL |
Time to result | 3h at RT |
Calibration | NIBSC (86/500) value (IU/mL) = 0,01 x HTRF hIL2 value (pg/mL) |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL2] (pg/mL) | CV |
1 | 75 | 7% |
2 | 2997 | 3% |
3 | 6136 | 2% |
Mean CV | 4% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL2] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 98 | 346 | 12% |
2 | 432 | 1576 | 12% |
3 | 1904 | 5911 | 10% |
Mean CV | 11% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Jurkat cells plated at 50 and 100 kcells/well were stimulated for 24 h with anti CD3 & and anti CD28 respectively prepared at 10 and 1 µg/mL. 16 µL of supernatants were transferred into a white detection plate (384 low volume) to be analyzed by the Human IL2 Assay.
In collaboration with CNRS - Scientific Presentations
Following T-cell activation and differenciation with HTRF reagents: IL-2, IFN-g and IL-22
In collaboration with Novartis - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF solutions for each cell type - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Discover no-wash cytokine assay solutions - Flyers
STING HTRF offer to bridge innate and adaptive immunity
cGAS-STING signaling pathway from A to Z - Brochures
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
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62hil02peg-62hil02peh - Product Insert
Manual IL2 (h) Kit / 62hil02pet
62hil02pet - Product Insert
Certificate of Analysis IL2 (h) Kit / 62HIL02PEG
62HIL02PEG Batch 06L - Quality Control Report
Certificate of Analysis IL2 (h) Kit / 62HIL02PEG
62HIL02PEG Batch 08D - Quality Control Report
Safety Data Sheet (DEU) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (h) Kit / 62HIL02PEG
62HIL02PEG - Safety Data Sheet
Certificate of Analysis IL2 (h) Kit / 62HIL02PEH
62HIL02PEH Batch 07F - Quality Control Report
Certificate of Analysis IL2 (h) Kit / 62HIL02PEH
62HIL02PEH Batch 07G - Quality Control Report
Safety Data Sheet (DEU) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (h) Kit / 62HIL02PEH
62HIL02PEH - Safety Data Sheet
Safety Data Sheet (DEU) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (h) Kit / 62HIL02PEJ
62HIL02PEJ - Safety Data Sheet
Certificate of Analysis IL2 (h) Kit / 62HIL02PET
62HIL02PET Batch 01X - Quality Control Report
Certificate of Analysis IL2 (h) Kit / 62HIL02PET
62HIL02PET Batch 01Y - Quality Control Report
Certificate of Analysis IL2 (h) Kit / 62HIL02PET
62HIL02PET Batch 01Z - Quality Control Report
Safety Data Sheet (DEU) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (h) Kit / 62HIL02PET
62HIL02PET - Safety Data Sheet
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