Human IFN gamma kit
No-wash kit to quantify released Human IFN gamma
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The HTRF human IL4 kit is designed for the quantification of human IL4 release in cell supernatant.
IL4 is expressed by T cells, NK cells, and mast cells, and is an important regulator of humoral and adaptative immunity. It stimulates activated B cells, and induces their differentiation into plasma cells and their class switch to immunoglobulin E, as well as promoting class II MHC expression. In immuno-oncology, IL4 induces the differentiation of naïve T cells into TH2 cells, which in turn produce IL4 and thus create a positive feedback loop. IL4 is also involved in the development of airway inflammation and allergic asthma.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-4 AlphaLISA kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL4 analysis.
Sample size | 16 µL |
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 8 pg/mL &29 pg/mL |
Range | 29 4,000 pg/mL |
Time to result | 2h at RT |
Calibration | NIBSC (88/656) value (IU/mL) = 0,012 x HTRF hIL4 value (pg/mL) |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL4] (pg/mL) | CV |
---|---|---|
1 | 50 | 7% |
2 | 1895 | 2% |
3 | 3598 | 3% |
Mean CV | 4% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL4] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 98 | 527 | 12% |
2 | 432 | 2061 | 7% |
3 | 1905 | 7265 | 6% |
Mean CV | 8% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF solutions for each cell type - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Manual IL4 (h) Kit / 62hil04peg-62hil04peh
62hil04peg-62hil04peh - Product Insert
Certificate of Analysis IL4 (h) Kit / 62HIL04PEG
62HIL04PEG Batch 02R - Quality Control Report
Certificate of Analysis IL4 (h) Kit / 62HIL04PEG
62HIL04PEG Batch 02S - Quality Control Report
Safety Data Sheet (DEU) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL4 (h) Kit / 62HIL04PEG
62HIL04PEG - Safety Data Sheet
Safety Data Sheet (DEU) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL4 (h) Kit / 62HIL04PEH
62HIL04PEH - Safety Data Sheet
Safety Data Sheet (DEU) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
Safety Data Sheet (ELL) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
Safety Data Sheet (ITA) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
Safety Data Sheet (SPA) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) IL4 (h) Kit / 62HIL04PEJ
62HIL04PEJ - Safety Data Sheet
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