Human GM-CSF kit
No-wash kit to quantify released Human GM-CSF
The HTRF human IL5 kit is designed for the quantification of human IL5 release in cell supernatant.
IL5 is mainly produced by TH2 cells upon IL4 activation, but also by eosinophils and mast cells. IL5 promotes B cell growth and stimulates immunoglobulin secretion. It also induces eosinophil activation, proliferation, and differentiation.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-5 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL5 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||15 pg/mL &71 pg/mL|
|Range||71 20,000 pg/mL|
|Time to result||2h at RT|
|Calibration||NIBSC (90/586) value (IU/mL) = 0,012 x HTRF hIL5 value (pg/mL)|
|Sample||Mean [IL5] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[IL5] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Can HTRF technology answer to different scientific questions? An academic with industrial standards point of view
In collaboration with CNRS - Scientific Presentations
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader
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