Human CCL2 (MCP1) kit
No-wash kit to quantify released Human CCL2 (MCP1)
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The HTRF human IL8 kit is designed for the quantification of human IL8 release in cell supernatant.
Also called CXCL8, IL8 is a chemokine mainly produced by macrophages, T cells, and neutrophils. IL8 acts as a chemoattractant for neutrophils, and promotes infiltration and activation at inflammation sites. IL8 is involved in several cancer types due to its ability to promote angiogenesis and cell proliferation, as well as to inhibit apoptosis.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-8 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL8 analysis.
Sample size | 16 µL |
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 6 pg/mL &32 pg/mL |
Range | 32 4,000 pg/mL |
Time to result | 1h at RT |
Calibration | NIBSC (89/520) value (IU/mL) = 0,01 x HTRF hIL8 value (pg/mL) |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL8] (pg/mL) | CV |
---|---|---|
1 | 58 | 9% |
2 | 314 | 4% |
3 | 2074 | 2% |
Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL8] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 78 | 273 | 2% |
2 | 376 | 1504 | 12% |
3 | 1818 | 5990 | 5% |
Mean CV | 6.3% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Sample | Dilution factor | [IL8] expected (pg/mL) | [IL8] detected (pg/mL) | Recovery |
1 | 1 | - | 2015 | - |
4.3 | 469 | 489 | 104% | |
7.4 | 271 | 292 | 108% | |
10.7 | 188 | 210 | 111% | |
Mean | 108% | |||
2 | 1 | - | 399 | - |
4.3 | 93 | 85 | 91% | |
7.4 | 54 | 56 | 103% | |
Mean | 97% |
The excellent % of recovery obtained from these experiments show the good linearity of the assay.
Sample | [IL8] added (pg/mL) | [IL8] expected (pg/mL) | [IL8] detected (pg/mL) | Recovery |
1 | 5,000 | 5,179 | 5,008 | 97% |
2 | 5,000 | 6,047 | 5,682 | 94% |
The same amount of recombinant cytokine was added to 2 different serum samples, and the set of responses obtained from a standard curve was compared to the calculated expected values. The ~ 100% of recovery found validates the sample matrix used for this assay.
THP1 cells plated at 100 kcells/well were incubated with increasing concentrations of JTE 607 for 18h, then stimulated for 3 h with 2 µg/mL LPS. 16 µL of supernatants were then transferred into a white detection plate (384 low volume) to be analyzed by the Human IL8 Assay.
PBMC plated at 50, 100, 200, and 400 kcells/well were stimulated for 3 h with increasing concentrations of LPS (0, 0.02, 0.2, 2 µg/mL). 16 µL of supernatants were then transferred into a white detection plate (384 low volume) to be analyzed by the Human IL8 Assay.
In collaboration with CNRS - Scientific Presentations
Celular High Throughput Screening assays using HTRF detection of cytokines
In collaboration with GSK - Scientific Presentations
Identification of synoviocyte activation inhibitors by parallel biomarker profiling.
In collaboration with Genomics institute of the Novartis Research Foundation - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert IL8 (h) Kit / 62HIL08PEG-62HIL08PEH
62HIL08PEG-62HIL08PEH - Product Insert
Product Insert IL8 (h) Kit / 62HIL08PET
62HIL08PET - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Discover no-wash cytokine assay solutions - Flyers
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
Neuroinflammation study combining hiPSC-derived astrocytes and HTRF
See published experiments and data demonstrating how immunoassays rise to the challenge of astrocyte studies in neuroinflammation research - Application Notes
On-demand webinar: Linking Neuroinflammation and Neurodegeneration
New insight into neuroinflammation research - Videos
Product Insert IL8 (h) Kit / 62HIL08PET-v6
62HIL08PET-v6 - Product Insert
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Safety Data Sheet (DEU) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL8 (h) Kit / 62HIL08PEG
62HIL08PEG - Safety Data Sheet
Safety Data Sheet (DEU) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL8 (h) Kit / 62HIL08PEH
62HIL08PEH - Safety Data Sheet
Safety Data Sheet (DEU) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
Safety Data Sheet (ELL) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
Safety Data Sheet (ITA) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
Safety Data Sheet (SPA) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
Safety Data Sheet (ENG-US) IL8 (h) Kit / 62HIL08PET
62HIL08PET - Safety Data Sheet
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