Human MBNL1 kit

The Human MBNL1 kit is designed for the rapid quantification of Muscleblind-like in cell lysates.
See more
  • Ready-to-use
  • Highly specific
  • Faster and more convenient than ELISA
The Human MBNL1 kit is designed for the rapid quantification of Muscleblind-like in cell lysates.
-
In stock

Overview

MBNL1 is involved in RNA-protein complexes with MBNL2 and DMPKRNA. In myotonic dystrophy 1 (DM1), known as a progressive neuromuscular disorder, aggregation of the mutant DMPKRNA with protein complexes has been associated to aberrant splicing of the insulin receptor. MBNL1 has been shown to bind CUG repeat sequences on RNA sequences.

Assay principle

Human MBNL1 is measured using a sandwich immunoassay involving two specific anti-MBNL1 antibodies respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of human MBNL1 present in the sample.

Assay Protocol

The MBNL1 assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of MBNL1. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Product Insert MBNL1 (h) Kit / 63ADK012PEG-63ADK012PEH

63ADK012PEG-63ADK012PEH - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

tab6