Human MBNL1 kit HTRF®

The Human MBNL1 kit is designed for the rapid quantification of Muscleblind-like in cell lysates.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Human MBNL1 kit is designed for the rapid quantification of Muscleblind-like in cell lysates.


MBNL1 is involved in RNA-protein complexes with MBNL2 and DMPKRNA. In myotonic dystrophy 1 (DM1), known as a progressive neuromuscular disorder, aggregation of the mutant DMPKRNA with protein complexes has been associated to aberrant splicing of the insulin receptor. MBNL1 has been shown to bind CUG repeat sequences on RNA sequences.



Assay principle

Human MBNL1 is measured using a sandwich immunoassay involving two specific anti-MBNL1 antibodies respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of human MBNL1 present in the sample.
MBNL1 kit principle

Assay Protocol

The MBNL1 assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of MBNL1. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
MBNL1 kit protocol

Product Insert MBNL1 (h) Kit / 63ADK012PEG-63ADK012PEH

63ADK012PEG-63ADK012PEH - Product Insert

HTRF Product Catalog

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A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

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Plate Reader Requirement

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