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Human PCSK-9 kit HTRF®

The Human PCSK-9 kit is designed for the rapid quantification of Proprotein convertase subtilisin/kexin type 9 in cell supernatants.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Human PCSK-9 kit is designed for the rapid quantification of Proprotein convertase subtilisin/kexin type 9 in cell supernatants.
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Overview

PCSK-9 (proprotein convertase subtilisin/kexin type 9) is a member of the proprotein convertase family that plays an important role in lipoprotein homeostasis. PCSK9 binds to the receptor for low-density lipoprotein particles (LDLR) within extracellular fluid, triggering ingestion of LDL-particles into cells. PCSK9 blocking can lower blood LDL-particle concentrations. The PCSK-9 kit is designed for the detection of proprotein convertase subtilisin/kexin type 9 directly in cell supernatants.

Benefits

  • VALIDATED ON LIVER CELLS
  • SELECTION OF PCSK-9 INHIBITORS

Assay principle

Human PCSK-9 is measured using a sandwich immunoassay involving two specific human PCSK-9 antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of PCSK-9 present in the sample.
PCSK-9 kit principle

Assay Protocol

The PCSK-9 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 2-hour incubation period at RT.
PCSK-9 kit protocol

Product Insert PCSK-9 (h) Kit / 63ADK050PEG-63ADK050PEH

63ADK050PEG-63ADK050PEH - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Plate Reader Requirement

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