cGMP kit
cGMP for accurate assessment in cells and for PDE
Human PCSK-9 kit
The Human PCSK-9 kit is designed for the rapid quantification of Proprotein convertase subtilisin/kexin type 9 in cell supernatants.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
PCSK-9 (proprotein convertase subtilisin/kexin type 9) is a member of the proprotein convertase family that plays an important role in lipoprotein homeostasis. PCSK9 binds to the receptor for low-density lipoprotein particles (LDLR) within extracellular fluid, triggering ingestion of LDL-particles into cells. PCSK9 blocking can lower blood LDL-particle concentrations. The PCSK-9 kit is designed for the detection of proprotein convertase subtilisin/kexin type 9 directly in cell supernatants.
Assay principle
Human PCSK-9 is measured using a sandwich immunoassay involving two specific human PCSK-9 antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of PCSK-9 present in the sample.
Assay Protocol
The PCSK-9 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure.
This protocol requires a single 2-hour incubation period at RT.
HTRF assays with iPSC derivated cell types
In collaboration with Cellular Dynamics International and BMG labtech - Scientific Presentations
Product Insert PCSK-9 (h) Kit / 63ADK050PEG-63ADK050PEH
63ADK050PEG-63ADK050PEH - Product Insert
Cisbio Product Catalog 2019
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides