Mouse IL1 beta kit
No-wash kit to quantify released Mouse IL1 beta
Human PCSK-9 kit HTRF®
The Human PCSK-9 kit is designed for the rapid quantification of Proprotein convertase subtilisin/kexin type 9 in cell supernatants.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
PCSK-9 (proprotein convertase subtilisin/kexin type 9) is a member of the proprotein convertase family that plays an important role in lipoprotein homeostasis. PCSK9 binds to the receptor for low-density lipoprotein particles (LDLR) within extracellular fluid, triggering ingestion of LDL-particles into cells. PCSK9 blocking can lower blood LDL-particle concentrations. The PCSK-9 kit is designed for the detection of proprotein convertase subtilisin/kexin type 9 directly in cell supernatants.
Benefits
- VALIDATED ON LIVER CELLS
- SELECTION OF PCSK-9 INHIBITORS
Assay principle
Human PCSK-9 is measured using a sandwich immunoassay involving two specific human PCSK-9 antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of PCSK-9 present in the sample.
Assay Protocol
The PCSK-9 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure.
This protocol requires a single 2-hour incubation period at RT.
Product Insert PCSK-9 (h) Kit / 63ADK050PEG-63ADK050PEH
63ADK050PEG-63ADK050PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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