Human Frataxin
Human Frataxin quantification in cell lysates
Human SMN kit HTRF®
This HTRF kit is designed for the rapid detection of human SMN produced by cells.
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Ready-to-use
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Low sample consumption
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Faster and more convenient than ELISA
Overview
SMN (Survival of motor neuron) is a 38-kD protein that is ubiquitously expressed in both the cytoplasm and nucleus. SMN plays an important role in assembling snRNP particles and is involved in RNA editing and transport. A lower expression of SMN has been correlated to the onset of Spinal Muscular Atrophy (SMA), an autosomal recessive disorder that is the leading hereditary cause of infant mortality, characterized by a loss of motoneurons in the spinal cord. The human SMN kit is designed for the detection of survival motor neuron protein directly in cell lysates.
Benefits
- SPINAL MUSCULAR ATROPHY RESEARCH
Assay principle
Human SMN is measured using a sandwich immunoassay involving two specific human SMN antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of SMN present in the sample.
Assay Protocol
The Human SMN assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of SMN. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Product Insert SMN (h) Kit / 63ADK033PEG-63ADK033PEH
63ADK033PEG-63ADK033PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
See how researchers boost their gene therapy studies with PerkinElmer technologies - Application Notes
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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