Human IL6 kit
No-wash kit to quantify released Human IL6
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The human STING WT binding assay is designed to select and characterize compounds that specifically bind human STING protein.
A fast and easy way to identify new binders to human STING WT (R232).
Stimulator of interferon genes (STING), also known as transmembrane protein 173 (TMEM173), is a protein playing a major role in innate immunity. Upon intracellular cytosolic DNA release from pathogens such as viruses and bacteria, 2’-3’cGAMP binds to STING protein and triggers the secretion of type 1 interferon. Identifying new STING ligands is therefore a way to control immunity and fight autoinflammatory diseases.
The HTRF STING binding assay is a competitive assay format which uses d2-labeled STING ligand, a 6His tagged human STING protein, and an anti 6His Cryptate-labeled antibody. Your compound competes with the STING ligand-d2 and thereby prevents FRET from occurring.
The Human STING binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate, the human His-tagged STING protein is then added followed by the dispensing of the HTRF reagents: the anti 6His antibody labeled with Terbium cryptate and the STING ligand labeled with d2. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.
STING ligand-d2 - Kd | 15 nM |
STING ligand-d2 - Concentration | 15 nM |
2’3’ cGAMP Standard - IC50 | 5 nM |
2’3’ cGAMP Standard - Ki | 2.5 nM |
Signal to Background | 12.4 |
Various compounds known as STING ligands were added to the assay. 2’-3’ cGAMP (assay standard), cyclic di-AMP, and cyclic di-GMP display the right potency in good correlation with the literature. DMXAA, a non-specific compound, also well-known mouse STING binder, has no effect on the assay, confirming the human specificity of the kit.
STING, for STimulator of INterferon Genes, is a cytoplasmic homodimeric protein localized in the endoplasmic reticulum which plays an essential role in innate immunity. Upon pathogen infection or mitochrondrial shrinking during apoptosis, floating dsDNAs bind and activate a DNA sensor, the cyclic GMP-AMP synthase (cGAS). Activated cGAS leads to the production of 2’-3’cGAMP, a cyclic dinucleotide, which then binds to STING proteins. In turn, phosphorylated STING interacts with TANK-binding-kinase-I (TBK1), leading to the recruitment and activation of the active interferon regulatory factor (IRF3) dimer. Nuclear translocation of the IRF3 dimer leads to the transcription of genes encoding IFN-α/β. In addition, the STING pathway controls NF-κB dependent inflammatory cytokine expression. As a negative feedback loop, the DNA-stimulated cGAS-STING-TBK1 pathway also triggers STING protein degradation through p62 SQSTM1 associated autophagy to switch off IFNb production.
STING HTRF offer to bridge innate and adaptive immunity
cGAS-STING signaling pathway from A to Z - Brochures
Novel HTRF platform to delineate STING pathway
Explore the whole STING pathway with a single technology - Posters
Product Insert Bioch. STING (h) kit / 64BDSTGPEG-64BDSTGPEH
64BDSTGPEG-64BDSTGPEH - Product Insert
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Webinar: Keeping cancer in check : Innate immunity and checkpoint inhibitors
Featuring a panel of experts - Videos
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Safety Data Sheet (DEU) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (ELL) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (ITA) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (SPA) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Bioch. STING (h) kit / 64BDSTGPEG
64BDSTGPEG - Safety Data Sheet
Safety Data Sheet (DEU) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
Safety Data Sheet (ELL) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
Safety Data Sheet (ITA) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
Safety Data Sheet (SPA) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Bioch. STING (h) kit / 64BDSTGPEH
64BDSTGPEH - Safety Data Sheet
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