Human TNFalpha/TNFR1 binding kit HTRF®

Associated products

The Human TNFalpha/TNFR1 binding kit is designed to identify human TNFalpha/TNFR1 inhibitors.

No-wash
All inclusive kit
Validated with reference Ab

Download the protocols

The Human TNFalpha/TNFR1 binding kit is designed to identify human TNFalpha/TNFR1 inhibitors.

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Overview

Deregulation of the tumor necrosis factor (TNF) plays an important role in the

initiation and perpetuation of chronic inflammation and has been implicated in the development of various autoimmune diseases. TNFalpha inhibitors are successfully used for the treatment of several diseases, such as rheumatoid arthritis and psoriasis.

Benefits

STUDY AUTOIMMUNE DISEASES
DISCOVER TNF-BLOCKING DRUGS
KD DETERMINATION

Assay principle

The HTRF TNFα/TNFR1 Binding kit is designed to measure the interaction between TNFα and TNFR1 proteins. Without treatment, TNFa binds to TNFR1, and the binding of each detection antibody to its respective tagged protein generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the TNFa/TNFR1 interaction is disrupted and the HTRF signal decreases.

HTRF human TNFα/TNFR1 binding kit assay principal

Assay protocol

The Human TNFalpha/TNFR1 binding kit can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The Tag1-TNFalpha protein and Tag2-TNFR1 protein are then added, followed by the dispensing of the HTRF reagents: the Tag1 antibody labeled with europium cryptate and the Tag2 XL665 reagent. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.

Inhibitory effects of TNFalpha/TNFR1 blockers

The inhibitory effects of small molecule unlabelled TNFR1 and TNFalpha, human anti-TNFalpha antibody (Adalimumab), and mouse anti-TNFR1 were tested. No inhibition was observed using an anti TNFR2 antibody, confirming the specificity of the kit for the study of TNFalpha/TNFR1 interaction.

Compound screening on TNFalpha/TNFR1 binfing kit

Best practices for pharmacological characterization of PPI inhibitors

Easy pharmacological characterization of PPI modulators. - Technical Notes

HTRF assays handle low- to high affinity protein-protein interactions

Deciphering low- and high affinity interactions - Application Notes

Nuclear receptor ligand identification with HTRF

Monitoring nuclear receptor binding with HTRF assays - Application Notes

HTRF addresses large protein-protein interaction complexes

Challenge large complexes with HTRF assays - Application Notes

HTRF PPI your dream assay served on a plate

Sandwiches aren't just for eating - Infographics

A brief history of Protein-Protein Interactions

How well do you know PPI? - Infographics

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Virology research solutions using HTRF Protein-Protein Interaction assays

See how peer researchers challenge the viral life cycle with PPI assays - Application Notes

Product Insert TNFa/TNFR1 (h) Kit / 64BDTNFPEG-64BDTNFPEH

64BDTNFPEG-64BDTNFPEH - Product Insert

autoimmune diseases therapeutic approaches

Complete autoimmunity review in a single White Paper - Guides

Autoimmune diseases and therapeutic strategies

Check out our new guide featuring valuable insights on autoimmunity - Guides

Assays for autoimmunity research

Advance your research on autoimmune diseases - Flyers

Safety Data Sheet (DEU) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (ELL) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (ITA) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (SPA) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (ENG-US) TNFa/TNFR1 (h) Kit / 64BDTNFPEG

64BDTNFPEG - Safety Data Sheet

Safety Data Sheet (DEU) TNFa/TNFR1 (h) Kit / 64BDTNFPEH