Human TNFalpha/TNFR1 binding kit HTRF®

Associated products

The Human TNFalpha/TNFR1 binding kit is designed to identify human TNFalpha/TNFR1 inhibitors.

No-wash
All inclusive kit
Validated with reference Ab

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The Human TNFalpha/TNFR1 binding kit is designed to identify human TNFalpha/TNFR1 inhibitors.

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Overview

Deregulation of the tumor necrosis factor (TNF) plays an important role in the

initiation and perpetuation of chronic inflammation and has been implicated in the development of various autoimmune diseases. TNFalpha inhibitors are successfully used for the treatment of several diseases, such as rheumatoid arthritis and psoriasis.

Benefits

STUDY AUTOIMMUNE DISEASES
DISCOVER TNF-BLOCKING DRUGS
KD DETERMINATION

Assay principle

The HTRF TNFα/TNFR1 Binding kit is designed to measure the interaction between TNFα and TNFR1 proteins. Without treatment, TNFa binds to TNFR1, and the binding of each detection antibody to its respective tagged protein generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the TNFa/TNFR1 interaction is disrupted and the HTRF signal decreases.

HTRF human TNFα/TNFR1 binding kit assay principal

Assay protocol

The Human TNFalpha/TNFR1 binding kit can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The Tag1-TNFalpha protein and Tag2-TNFR1 protein are then added, followed by the dispensing of the HTRF reagents: the Tag1 antibody labeled with europium cryptate and the Tag2 XL665 reagent. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.

Inhibitory effects of TNFalpha/TNFR1 blockers

The inhibitory effects of small molecule unlabelled TNFR1 and TNFalpha, human anti-TNFalpha antibody (Adalimumab), and mouse anti-TNFR1 were tested. No inhibition was observed using an anti TNFR2 antibody, confirming the specificity of the kit for the study of TNFalpha/TNFR1 interaction.