MAb Anti-phospho p53-Eu cryptate
Anti;phospho p53;Eu cryptate to detect phospho;p53;Ser15.
This HTRF kit is designed for the rapid detection of human TREM-2 released by cells.
The kit is designed for the rapid detection of TREM-2 (also known as Triggering Receptor Expressed on Myeloid Cells 2) in cell supernatant and whole cells. The TREM family of receptors regulates the activity of various cell types of the immune system, including neutrophils, monocyte/macrophages, microglia, and dendritic cells. Soluble TREM-2 has been detected in human cerebrospinal fluid (CSF), where it was found to be elevated in patients with multiple sclerosis and other inflammatory neurological conditions in comparison to patients without inflammatory neurologic disorders.
Three different cellular densities of THP-1 cells were seeded in a 96-well plate and differentiated by adding 100nM of PMA, causing the cells to become adherent. The final volume was 200µl in each well. The stimulation was done for 72 hours at 37°C.
Cell supernatant was then collected for the soluble TREM2 quantification. Adherent cells were washed 3 times with complete cell culture medium, and the final supernatant was collected for soluble TREM2 detection. Cells were finally incubated for 24 hours at 37°C and a final sample was collected. For each sample, several dilutions were assessed to ensure a correct concentration interpolation.
The TREM2 receptor is highly expressed and cleaved after PMA induced THP-1 differentiation. The washing treatment enables the removal of remaining soluble TREM2. A 24 hour additional incubation enables the detection of newly released soluble TREM2.
Get the brochure about technology comparison. - Brochures
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Discover this infographic design on neurodegenerative diseases - Infographics
See published experiments and data demonstrating how HTRF rises to the challenge of studying microglia in neuroinflammation research. - Application Notes
New insight into neuroinflammation research - Videos
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader