Hyaluronic Acid kit HTRF®

The Hyaluronic Acid kit is designed for the rapid detection of HA in cell supernatants.

Ready-to-use
Highly specific
Low sample consumption

Download the protocols

The Hyaluronic Acid kit is designed for the rapid detection of HA in cell supernatants.

Size

Cat. number selected :

Quantity

Overview

Hyaluronic Acid (HA), also called hyaluronan, is an important component of the extracellular matrix contributing significantly to cell proliferation and migration. HA may also be involved in the progression of some malignant tumors. HA acts as a promoter of early inflammation, which is crucial to the whole skin wound-healing process. The Hyaluronic Acid kit is designed for a rapid detection of HA in cell supernatants.

Benefits

STUDY OF LIVER FIBROSIS
INFLAMMATION RESEARCH

Assay principle

Hyaluronic Acid is measured using a sandwich immunoassay involving Hyaluronic Acid Binding Protein (HABP) labelled with terbium Cryptate (donor) and HABP labelled with d2 (acceptor). The intensity of the signal is proportional to the concentration of Hyaluronic Acid present in the sample.

Assay Protocol

Cells are plated and stimulated in the same 96-well culture plate. Cell supernatants are then transferred into the assay plate for the detection of Hyaluronic Acid by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The binding buffer is required to enhance the detection of Hyaluronic Acid on samples. The HABP labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Biomarker and Cell Signaling Assays for Fibrosis and NASH

HTRF solutions for NASH - Flyers

Webinar: NASH - Fibrosis Research and Drug Discovery

Featuring a panel of experts - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Insight into fibrosis development and signaling pathways

A comprehensive overview of fibrosis development - Guides

Advance your research on Fibrosis

Kits and reagents for Fibrosis research - Flyers

Product Insert Hyalur acid (h) Kit / 63ADK089PEG-63ADK089PEH

63ADK089PEG-63ADK089PEH - Product Insert

Product Insert Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Product Insert

Product Insert Hyalur acid (h) Kit / 63ADK089PEH

63ADK089PEH - Product Insert

Safety Data Sheet (DEU) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (ELL) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (ITA) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (SPA) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (ENG-US) Hyalur acid (h) Kit / 63ADK089PEG

63ADK089PEG - Safety Data Sheet

Safety Data Sheet (DEU-) Hyalur acid (h) Kit / 63ADK089PEH