Glucagon Kit
Glucagon quantification in cell supernatant
Insulin Ultra-Sensitive Assay kit HTRF®
The Insulin Ultra-Sensitive kit is designed for the quantification of insulin in low-concentration samples of cell supernatant. An excellent tool in diabetes research.
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Ease-of-use
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No-wash
Overview
One hurdle faced by researchers seeking to quantify insulin is the high number of islets often required per assay. Designed for enhanced sensitivity, this kit is intended for the quantification of insulin in low-concentration samples. The kit is ideal for the detection of basal insulin levels and insulin released from mouse pancreatic islets.
Benefits
- HIGH SENSITIVITY
- DIABETES RESEARCH
Assay principle
Insulin is measured using a sandwich immunoassay of two monoclonal antibodies, one labelled with Cryptate (donor) and the other with XL665 (acceptor). The intensity of the FRET signal obtained is proportional to the concentration of insulin in the sample.
Assay protocol
10 µL of cell supernatants are supplemented with 5 µL of acceptor-labelled antibodies and 5 µL of donor-labelled antibodies (or 10 µL of pre-mixed reagents for a single dispensing step). The mix is then incubated from 4h to overnight at RT and results are read on an HTRF compatible reader.
Correlation with leading RIA
The HTRF Ultra Sensitive insulin assay showed an excellent correlation to the leading RIA on mouse pancreatic islet supernatants. With a slope of 0.946, the regression line was close to the ideal line, demonstrating the assay's reliability.
Validation on mouse pancreatic islets
Pancreatic islets were isolated from C57BL/6N male mice. The islets were dispensed into tubes with KRB buffer containing either 2.8 mM or 16.7 mM glucose (5 islets/tube, 6 tubes/condition). After 10 minutes or 30 minutes of incubation, the supernatants* were collected and the insulin concentrations measured using the HTRF Ultra-sensitive insulin kit reagents.
*Samples were kindly provided by S. Dalle and M. Ravier’s research team: Physiopathology of pancreatic beta-cells, Institute for Functional Genomics (IGF), Montpellier, France.
The next level in Insulin quantification assays
Compare easily the 3 Cisbio kits for insulin quantification - Guides
Product Insert Insulin U Sens Kit / 62IN2PEG-62IN2PEH
62IN2PEG-62IN2PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
A guide to help you in your diabetes projects
A comprehensive listing of Type II diabetes pathways - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (ELL) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (ITA) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (SPA) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Insulin U Sens Kit / 62IN2PEG
62IN2PEG - Safety Data Sheet
Safety Data Sheet (DEU) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Safety Data Sheet (ELL) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Safety Data Sheet (ITA) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Safety Data Sheet (SPA) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Insulin U Sens Kit / 62IN2PEH
62IN2PEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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