Human S100A12 protein (Calgranulin C) in stool ELISA kit

The Inflamark F-INFL-EL-US quantifies the human S100A12 protein in stool. This kit is availble for research use only, not for use in diagnostic procedures.

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The Inflamark F-INFL-EL-US quantifies the human S100A12 protein in stool. This kit is availble for research use only, not for use in diagnostic procedures.

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Overview

Biomarqueur pour l'inflammation de l'intestin Investigation clinique des patients atteints de la maladie de Crohn

Investigation clinique des patients atteints de la colite ulcéreuse

Évaluation de manière non invasive des intestins endommagés chez les patients atteints de colite ulcéreuse

Différentiation entre la maladie inflammatoire de l'intestin et le syndrome du côlon irritable

Benefits

  • EXCELLENT REPRODUCIBILITY
  • ROBUST AND RELIABLE, AUTOMATION FRIENDLY

Assay principle: sandwich

Two monoclonal antibodies directed against the S100A12 protein are used for the sandwich. A first monoclonal antibody is immobilized on the microplate, while a second monoclonal antibody is conjugated to Horse-Radish peroxidase. After the second incubation, unfixed reagents are eliminated before the addition of the HRP substrate TMB. NB: prior to the assay, a faeces treatment and 1/50 predilution of the samples are required.

Assay procedure: 30 min at RT / shaking + 30 min at RT / shaking + 10 min at RT.

Stool Inflamark kit Flow Chart

Stool Inflamark kit Flow Chart

Features

Working range: 0.21 – 12 µg/g of stool

Sample: stool (Faeces treatment + predilution of samples 1/50) – 100 µL Functional sensitivity: 0.36 µg/g of stool

Normal values

PopulationnMedian
µg/g of stool
Healthy controls30<0.27
Crohn’s disease3014
Ulcerative colitis3014
Product list 2019

Immunoassays RIA and ELISA - Catalog

Catalogue produits 2019

Immuno-analyse RIA et ELISA - Catalog

Annual availability Schedule 2019

In-vitro RIA products - Availability calendar

Calendrier de mise a disposition 2019

Trousse pour radioimmunologie - Availability calendar

Safety Data Sheet (DEU) S100-US / F-INFL-EL-US

F-INFL-EL-US - Safety Data Sheet

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