Somatomedin C (IGF-I) immunoradiometric assay kit

The IGF1-US kit is an immunoradiometric assay for a measure of somatomedin C (IGF-I).

For Research Use Only, not for use in diagnostic procedures.

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The IGF1-US kit is an immunoradiometric assay for a measure of somatomedin C (IGF-I).

For Research Use Only, not for use in diagnostic procedures.

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In stock

Benefits

  • NO INTERFERENCE WITH BINDING PROTEINS
  • NO EXTRACTION NECESSARY

Assay principle: sandwich

Two monoclonal antibodies directed against two different antigenic sites of the IGF-I molecule are used for the sandwich. The first one is adsorbed onto the tube walls. The second one, radiolabeled with iodine-125, is used as the tracer. A predilution of the samples (1/31) with an acidic solution is required to strip the carrier protein (BP). This carrier protein is then saturated by IGF-II. The use of IGF-II molecules in the assay avoids any reassociation between IGF-I and the carrier protein (IGF-BP).

Assay procedure: 2h at RT / shaking + 2h at RT / shaking.

Features

Working range: 1 – 1000 ng/mL (IRS 87/518)

Sample: serum or plasma – 25 µL - predilution required (1/31) Analytical sensitivity: 1 ng/mL

Product list 2019

Immunoassays RIA and ELISA - Catalog

Catalogue produits 2019

Immuno-analyse RIA et ELISA - Catalog

Annual availability Schedule 2019

In-vitro RIA products - Availability calendar

Calendrier de mise a disposition 2019

Trousse pour radioimmunologie - Availability calendar

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