HTRF active GLP-1 kit is a rapid sandwich assay for accurate quantification of the glucagon-like peptide-1 active forms, GLP-1(7-36)NH2 and GLP-1(7-37) on cell supernatants. The simple and robust procedure gives increased throughput compared to ELISA. The extended dynamic range allows crude sample to be analyzed with no need of a pre-dilution step.
GLP-1 has become a key biomarker in treatment of type 2 diabetes. The main actions of GLP-1 are stimulation of insulin secretion and inhibition of glucagon secretion and food intake. In vivo, the active forms are rapidly degraded into inactive forms (9-36)NH2 and (9-37) by the dipeptidyl peptidase IV (DPP-IV).
Active GLP-1 assay complements other HTRF technology based assays in Cisbio’s offering for diabetes investigation, such as insulin, cortisol, cAMP and GLP-1 receptor ligand binding assay.