Adenosin A2A Receptor cell-based binding assay

Immune checkpoint assay to identify adenosine A2A receptor checkpoint inhibitors

Extracellular adenosine, present in the tumor micro-environment (TME), has been identified as a fundamental element in immune response regulation.

Cancer cell proliferation generates local hypoxia in the TME,  leading to secretion of adenosine by the surrounding cells. In this context, the interaction between adenosine and its A2A receptor can be stimulatory or inhibitory, depending on the state of the TME and the immune system.

Our range offers everything you need to set up your cell-based binding assay: labelled reference lignad, labelled frozen cells, and buffers. They can be used to set up saturation biding assays (Kd) or competitive binding assays (Ki).

Download Adenosine A2A assay protocol

Assay principle

The Tag-lite® Adenosin A2A Receptor Ligand Binding Assay is  a homogeneous alternative to radio ligand binding assays for HTS and compound profiling.

It is suitable for both saturation binding assays (Kd) and competitive binding assays  (Ki). At equilibrium, the fraction of labeled ligand bound to the receptor is proportional to the FRET signal recorded. From this signal, binding affinities can be calculated.

Saturation binding assay (Kd determination)

The Tag-lite® Adenosin A2A Receptor Ligand Binding Assay is  a homogeneous alternative to radio ligand binding assays for HTS and compound profiling.

It is suitable for both saturation binding assays (Kd) and competitive binding assays  (Ki). At equilibrium, the fraction of labeled ligand bound to the receptor is proportional to the FRET signal recorded. From this signal, binding affinities can be calculated.


How to implement a saturation binding
assay using Tag-lite® technology

Competitive binding assay (Ki determination)

Measurement of a compound's dissociation constant.


How to implement a competitive binding
assay using Tag-lite® technology

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