This assay is designed to measure the endogenous expression level of alpha-tubulin in cells and tissues to normalize data obtained on the phospho- and/or total protein(s) of interest.
Due to its essential role in the cytoskeleton of cells, alpha-tubulin is ubiquitously and constitutively expressed in every tissue. Moreover, its amino acid sequence is highly conserved and is thus identical in almost all species. These characteristics make it one of the most commonly used housekeeping proteins. Its concentration is proportional to cell number and total protein concentration, and it is therefore used as an internal control for data normalization to correct for signal changes caused by experimental variability (e.g. number of cells remaining in the culture plate after treatment or lysis efficacy).
The low sample volume of 4 µL and the compatibility with Cisbio’s lysis buffers #1, #2, #3 and #4 enables a multi-parametric analysis with the detection of alpha-tubulin in parallel with the phospho- and/or total protein(s) on the same lysate.