Amyloid β1-40 Assay Kit

Amyloid β1-40 and β1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, β and γ secretase, which cleave the amyloid precursor protein. Cisbio Bioassays has developed an assay for the measurement of amyloid β1-40 peptides, based on our proprietary HTRF® technology. This assay can be rapidly performed, easily miniaturized, and is highly amenable to automation.

Assay principle

The HTRF Amyloid β1-40 assay is a sandwich immunoassay using two monoclonal antibodies which recognize different epitopes within the β1-40 peptide. One antibody is labeled with Eu3+-Cryptate and the other with XL-665. The assay can be used with whole cells or cell supernatant.

The Amyloid β1-40 peptide assay can be performed in a single microplate, from cell stimulation through to HTRF signal detection. The quantification process involves the addition of the two specific antibody-conjugates which can be pre-mixed, enabling a single reagent addition protocol. This simplified protocol can be miniaturized and is suitable for HTS.

Product specifications

Detection limit: 13.64 pg/mL
Dynamic range: 25 to 1600 pg/mL
Sample compatibility: Cell-based or cell supernatants
Specificity: No cross-reaction with Amyloid β 1-42 and β 1-43

Amyloid β1-40 peptide standard curve was performed using the assay protocol outlined in the product insert.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human Amyloid beta 1-40 kit - 500 tests62B40PEG
Human Amyloid beta 1-40 kit - 10,000 tests62B40PEH
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Companion products

DescriptionCat. noProduct insertMSDS
Human Amyloid beta 1-40 kit standard62B40CDA
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Diluent 1 - 20 ml62DL1DDD

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