Cleaved PARP assay as a relevant marker of cell apoptosis
Cisbio's cell-based homogeneous HTRF® kit for cleaved PARP (Asp214) enables the specific and quantitative detection of endogenous levels of the human, mouse and monkey large fragment (89 kDa) of PARP-1. With its central position downstream from caspases 3 and 7, both activated by extrinsic and intrinsic apoptosis pathways, cleaved PARP-1 represents a pertinent marker of cells undergoing apoptosis. It also comes as a useful complement to the HTRF phospho-BAD assay, used to monitor the activation of the Bcl-2 pro-apoptotic proteins of the intrinsic pathway. Dysregulated apoptosis plays a role in various diseases involving insufficient cell death such as cancer, autoimmunity and persistent infections, whereas excessive apoptosis can contribute to neurodegeneration, and ischaemia. The kit's simple add-and-read protocol eliminates all wash steps for a faster analysis and easy miniaturization, while maintaining a high-quality specific output and offering enhanced convenience over ELISA or WB.