There is substantial research indicating that breast cancer tissues contain the enzymes necessary to synthesize estradiol (17β-estradiol), a steroid hormone which plays an important role in this disease. Two principal pathways are involved in the formation of estradiol in breast cancer tissues: the aromatase pathway, which transforms androgens into estrogens, and the sulfatase pathway, which converts estrone into estradiol via the 17β- HSD1 enzyme. Based on HTRF® technology, the Estradiol kit is a rapid and highly sensitive assay for measuring estradiol in whole cells, cell supernatants or biochemical enzyme assays.
Our Technology HTRF
HTRF® technology principle:
Truly homogeneous functional assays
FRET (Fluorescence Resonance Energy Transfer) is based on the transfer of energy between two fluorophores – a donor and an acceptor – when in close proximity. Molecular interactions between biomolecules can be assessed by coupling each partner with a fluorescent label and by detecting the level of energy transfer.