Cisbio, Codolet, France
This technical note reviews the general principles and steps in developing a protein:protein interaction assay using HTRF PPi reagents from Cisbio Bioassays. In an HTRF protein:protein interaction assay, one protein is labeled (directly or indirectly) with the donor, and
the other protein is labeled (directly or indirectly) with the acceptor. When the two proteins interact, the donor molecule is brought within proximity of the acceptor molecule. Excitation of the donor will result in signal generation proportional to the binding of proteins.