HTRF 384-well low volume white plate

These plates have been specifically designed by Cisbio.
Cisbio’s 384-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells.
These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy.

 

Check our 384-w & 1536-w microplate recommendations

 

Plate characteristics

Format 384 well
Color White
Bottom Opaque
Maximum volume / well 32 µL
Recommended working volume / well 20-26 µL
Culture NOT culture treated
Lid Compatible
Sealer Compatible (and recommended)
Reader settings 384 half-area
Validation Inter-batch & intra-batch

 

HTRF 96-well low volume white plate characteristics plate

HTRF 96-well low volume white plate characteristics plate

HTRF 96 well low volume white plate characteristics plate side view

HTRF 96-well low volume white plate characteristics plate

 

Plate validation

HTRF assays in HTRF 384-well plate and in Greiner 384-well plate

Assay reagents were dispensed side-by-side in an HTRF 384-well low volume plate and in a Greiner 384-well low volume plate. As shown below, similar results were obtained, whether the assay was performed in Greiner plates or in HTRF plates.

1. cAMP Gs dymanic kit

HTRF 384 well plate standard curve of camp gs assay kit

A standard curve was prepared and dispensed in the two different plates: 10 µL of each standard, 5 μL of cAMP-d2 and 5 μL of anti cAMP- cryptate were added into the wells of each plate. HTRF signal was recorded after a 1 hour incubation at RT.

2. Mouse CCL2 kit

HTRF 384 well plate standard curve of Mouse CCL2 assay kit

A standard curve was prepared and dispensed in the two different plates: 16 µL of each standard and 4 µL of pre-mixed Anti-CCL2 antibodies were added into the wells of each plate. HTRF signal was recorded after an overnight incubation at RT.

3. Total SMAD3 kit

HTRF 384 well plate signal total SMAD3

16 µL of various amount of untreated NIH3T3 cells lysed with the supplemented lysis buffer #4, were dispensed into the wells of the two different plates, 4 µL of of pre-mixed Anti-SMAD3 antibodies were added into the wells of each plate. HTRF signal was recorded after an overnight incubation at RT.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
HTRF 384 well low volume plate - 25 plates66PL384025
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HTRF 384 well low volume plate - 100 plates66PL384100
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