HTRF ALPHA-TUBULIN HOUSE KEEPING KIT: Matching versatility with dynamic range for unprecedented performance


Cisbio, Codolet, France

Alpha-tubulin is a component of microtubules. Due to its essential role in the cytoskeleton, this protein is ubiquitously and constitutively expressed in every tissue. Moreover, its amino acid sequence is highly conserved and is thus identical in almost all species. These characteristics make it one of the most commonly used housekeeping proteins.
The HTRF Alpha-Tubulin Housekeeping kit is based on a homogeneous TR-FRET immunoassay format and measures the endogenous level of α-tubulin in cell and tissue lysates.
This application note highlights the unmatched versatility of the HTRF Alpha-Tubulin assay kit and provides a convincing demonstration of its applicability to a variety of sample and experimental conditions. Compatibility with all Cisbio lysis buffers, species cross-reactivity, as well as correlation with BCA Total Protein assay are presented below for the reader’s review.

Phosphorylated proteins

Phospho-4EBP1 (Thr37/46) Cellular Assay KitPhospho-ACC (Ser79) Cellular Assay KitPhospho- & total AKT 1/2/3, Phospho- & total pan AKT Cellular Assay KitsPhospho-AMPK (Thr172) & Total AMPK Cellular Assay Kits Phospho-ATF2 (Thr71) Cellular Assay KitPhospho-β-Catenin (Thr41/Ser37/Ser33) & Total β-Catenin Cellular Assay Kits Phospho-Bad (Ser112) Cellular Assay Kit Phospho-BTK (Tyr223) & Total BTK Cellular Assay KitsPhospho-c-Jun (Ser63) Cellular Assay KitPhospho-c-RAF (Ser338) Cellular Assay KitPhospho-c-Src (Tyr419) and Total c-Src Assay KitsPhospho-CREB (Ser133) & Total CREB Cellular Assay KitsPhospho-EGFR (pan), Phospho-EGFR (Tyr1068) & Total EGFR Cellular Assay KitsPhospho-EIF2 alpha (Ser52) & Total EIF2 alpha Cellular Assay KitsPhospho-EIF4E (Ser209) Cellular Assay Kit Phospho-HER2 (Tyr1221/1222) & Total HER2 Cellular Assay KitsPhospho-ERK (Thr202/Tyr204), Advanced phospho-ERK (Thr202/Tyr204), and Total ERK1/2 Cellular Assay KitsPhospho-FGFR2 (Tyr653/654) & Total FGFR2 Cellular Assay KitsPhospho-FoxO1 (Ser256) and Total FoxO1 Assay KitsPhospho-GSK3β (Ser9), Phospho-GSK3α (Ser21), Total GSK3β & Total GSK3α Cellular Assay KitsPhospho-H2AX (Ser139) Cellular Assay KitPhospho-HER2 (Tyr1221/1222) & Total HER2 Cellular Assay KitsPhospho-HER3 (Tyr1289) & Total HER3 Cellular Assay KitsPhospho-Histone H3 (Ser10), Phospho-Histone H3 (Thr3) & Total Histone H3 Cellular Assay KitsPhospho-IKKβ (Ser177/181) & Total IKKβ Cellular Assay Kit Phospho-Insulin Receptor (Tyr1150/1151) & Total Insulin Receptor Cellular Assay KitsPhospho-IRF3 (Ser386) Cellular Assay KitPhospho-IRS1 (Ser307 mouse / Ser312 human) & Total IRS1 Cellular Assay KitsTotal IRS2 Cellular Assay KitPhospho-JNK (Thr183 / Tyr185) Cellular Assay Kit Phospho-LRRK2 (Ser935) & Total LRRK2 Cellular Assay KitsPhospho-MEK1 (Ser218/222), Phospho-MEK1/2 (Ser218/222), Phospho-MEK1 (Ser298) & Total MEK1 Cellular Assay KitsPhospho-MNK (Thr197/202) Cellular Assay KitPhospho-mTOR (Ser2448) Cellular Assay KitPhospho & Total NDRG1 Assay KitPhospho-NFkB (Ser536) & Total NFkB Cellular Assay KitsPhospho-p38 MAPK (Thr180/Tyr182) Cellular Assay KitPhospho-p53 (Ser15) & Total p53 Cellular Assay KitsPhospho-P70S6K (Thr389) Cellular Assay Kit Phospho-Phospholamban (Thr17) & total Phospholamban Cellular Assay KitsPhospho-S6RP (Ser235/236) Cellular Assay Kit Phospho-SLP-76 (Ser376) & total SLP-76 Cellular Assay KitsPhospho-SMAD1 (Ser463/465) & Total SMAD1 Cellular Assay KitsPhospho-SMAD3 (Ser423/425) & Total SMAD3 Cellular Assay KitsPhospho-STAT3 (Tyr705) & Total STAT3 Cellular Assay KitsPhospho-STAT5 (Tyr694) Cellular Assay KitPhospho-TAU (Ser202/Thr205) Cellular Assay KitPhospho-ULK1 (Ser757 mouse / Ser758 human) Assay KitPhospho-VASP (Ser157), Phospho-VASP (Ser239) & Total VASP Cellular Assay KitsPhospho-VEGFR2 (Tyr1175) Cellular Assay KitPhospho-YAP (Ser127) & Total YAP Cellular Assay KitsPhospho-ZAP-70 (Tyr319) & Total ZAP-70 Cellular Assay KitsAlpha-Tubulin Housekeeping Cellular KitSTAT1 phospho (Tyr701) & total assaysPhospho-TBK1 (Ser172) & Total TBK1 Assay KitsPhospho-PDGFR beta (Tyr751) Cellular Assay KitPhospho-STING (Ser366) Cellular KitPhospho-SHP2 (Tyr542) Cellular KitTotal STING Cellular KitPhospho-SHP1 (Tyr564) Cellular KitTotal SHP1 Cellular KitTotal SHP2 Cellular KitPhospho-FAK (Tyr397) Cellular KitTotal FAK Cellular KitPhospho-STAT6 (Tyr641) Cellular KitHuman Ki-67 HTRF assay kitHuman STING binding kitAlpha-Tubulin Housekeeping – standard