Cisbio Bioassays, Codolet, France
Cisbio offers a comprehensive line of HTRF phospho- and total protein assays to study posttranslational cellular modifications. These versatile, homogeneous assays are suitable for a variety of sample types, including primary cell lines and tumor xenografts. Combining an HTRF phospho-assay with its associated total protein assay enables researchers to accurately identify candidate molecules that regulate phosphorylation, as well as to characterize their mechanism of action by performing the appropriate data analysis.
This application note compares the use of HTRF phospho- and total protein assays with traditional Western blot for analyzing cell signaling pathways in tumor xenografts. The data demonstrates that HTRF is a faster, simpler, and more accurate technique for assessing protein phosphorylation and for revealing subtle drug-induced modulations than Western blotting.